Dose dependent neuroprotection of the noble gas argon after cardiac arrest in rats is not mediated by KATP-Channel opening

被引：41

作者：

Bruecken, Anne ^{[1
]}

Kurnaz, Pinar ^{[1
]}

Bleilevens, Christian ^{[1
]}

Derwall, Matthias ^{[1
]}

Weis, Joachim ^{[2
]}

Nolte, Kay ^{[2
]}

Rossaint, Rolf ^{[1
]}

Fries, Michael ^{[1
]}

机构：

[1] Univ Hosp RWTH Aachen, Dept Anaesthesiol, D-52074 Aachen, Germany

[2] Univ Hosp RWTH Aachen, Inst Neuropathol, D-52074 Aachen, Germany

来源：

RESUSCITATION | 2014年 / 85卷 / 06期

关键词：

Cardiopulmonary resuscitation; Hypoxia-ischaemia; Brain; Reperfusion injury; Argon; Neuroprotective agents; TRAUMATIC BRAIN-INJURY; IN-VITRO MODEL; NEONATAL ASPHYXIA; HYPOTHERMIA COMBINE; CEREBRAL-ISCHEMIA; POTASSIUM CHANNEL; XENON; PIGS; ANESTHESIA; NEURONS;

D O I：

10.1016/j.resuscitation.2014.02.014

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

Purpose: Argon at a dosage of 70% is neuroprotective when given 1 h after cardiac arrest (CA) in rats. In a rodent model, we investigated if the neuroprotective effects of argon are dose dependent and mediated by adenosine triphosphate dependent potassium (K-ATP) channels. Methods: Forty-seven male Sprague-Dawley rats were subjected to 7 min of CA and 3 min of cardiopulmonary resuscitation (CPR). In protocol I animals were randomized to receive either 70% or 40% argon ventilation 1 h after successful CPR or no argon-treatment. Animals of the second protocol also received 1 h of 70% argon ventilation or no argon treatment but were randomized to a group receiving the KATP channel blocker 5-hydroxydecanoate (5-HD). For all animals a neurological deficit score (NDS) was calculated daily for seven days following the experiment before the animals were killed and the brains harvested for histopathological analyses. Results: All animals survived. Control animals exhibited severe neurologic dysfunction at all points in time as measured with the NDS. Argon treated animals showed significant improvements in the NDS through all postoperative days in a dose dependent fashion. This was paralleled by a significant reduction in the neuronal damage index in the neocortex and the hippocampal CA 3/4 region. Administration of 5-HD neither abolished the positive effects on functional recovery nor on histopathologic changes observed in the argon group. Conclusion: Our study demonstrates a dose dependent neuroprotective effect of argon administration in this rodent model, which is not mediated via ATP dependent potassium channels. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：826 / 832

页数：7

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