A molecular scheme for Yersinia enterocolitica patho-serotyping derived from genome-wide analysis

被引:47
作者
Garzetti, Debora [1 ]
Susen, Rosa [1 ]
Fruth, Angelika [2 ]
Tietze, Erhard [2 ]
Heesemann, Juergen [1 ]
Rakin, Alexander [1 ]
机构
[1] Univ Munich, Max von Pettenkofer Inst Hyg & Med Microbiol, D-80336 Munich, Germany
[2] Robert Koch Inst, Wernigerode Branch, Div Enteropathogen Bacteria & Legionella, Natl Reference Ctr Salmonellae & Other Bacterial, Wernigerode, Germany
关键词
Yersinia enterocolitica; Molecular patho-serotyping; O-antigen; Genome-wide comparison; LIPOPOLYSACCHARIDE O-ANTIGEN; VIRULENCE GENES; PCR; IDENTIFICATION; BIOSYNTHESIS; BIOTYPES; SEQUENCE; CHAIN;
D O I
10.1016/j.ijmm.2013.10.007
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Yersinia enterocolitica is a food-borne, gastro-intestinal pathogen with world-wide distribution. Only 11 serotypes have been isolated from patients, with O:3, O:9, O:8 and O:5,27 being the serotypes most commonly associated with human yersiniosis. Serotype is an important characteristic of Y. enterocolitica strains, allowing differentiation for epidemiology, diagnosis and phylogeny studies. Conventional serotyping, performed by slide agglutination, is a tedious and laborious procedure whose interpretation tends to be subjective, leading to poor reproducibility. Here we present a PCR-based typing scheme for molecular identification and patho-serotyping of Y. enterocolitica. Genome-wide comparison of Y. enterocolitica sequences allowed analysis of the O-antigen gene clusters of different serotypes, uncovering their formerly unknown genomic locations, and selection of targets for serotype-specific amplification. Two multiplex PCRs and one additional PCR were designed and tested on various reference strains and isolates from different origins. Our genotypic assay proved to be highly specific for identification of Y. enterocolitica species, discrimination between virulent and non-virulent strains, distinguishing the main human-related serotypes, and typing of conventionally untypeable strains. This genotyping scheme could be applied in microbiology laboratories as an alternative or complementary method to the traditional phenotypic assays, providing data for epidemiological studies. (C) 2013 Elsevier GmbH. All rights reserved.
引用
收藏
页码:275 / 283
页数:9
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