Major Hepatectomy Induces Phenotypic Changes in Circulating Dendritic Cells and Monocytes

被引:11
作者
Efron, Philip A. [1 ]
Matsumoto, Tadashi [1 ]
McAuliffe, Priscilla F. [1 ]
Scumpia, Philip [1 ]
Ungaro, Ricardo [1 ]
Fujita, Shiro [1 ]
Moldawer, Lyle L. [1 ]
Foley, David [1 ]
Hemming, Alan W. [1 ]
机构
[1] Univ Florida, Coll Med, Dept Surg, Shands Hosp, Gainesville, FL 32610 USA
关键词
Inflammation; dendritic cell maturation; monocyte activation; hepatectomy; innate immunity; TOLL-LIKE RECEPTOR-2; CARDIOPULMONARY BYPASS; NEUTROPHIL ELASTASE; CYTOKINE PRODUCTION; BLOOD MONOCYTE; ACTIVATION; EXPRESSION; SEPSIS; SURGERY; LYMPHOCYTE;
D O I
10.1007/s10875-009-9291-y
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Patients undergoing major hepatectomy are at increased risk for post-operative morbidity and mortality, and changes in the phenotype of effector cells may predispose these patients to infectious sequelae. To better understand post-hepatectomy immune responses, peripheral blood from 15 hepatectomy patients was drawn immediately before and after liver resection and on post-operative days 1, 3, and 5. Circulating monocytes and dendritic cells were analyzed by flow cytometry for quantity, phenotype, activation status, human leukocyte antigen DR (HLA-DR) expression, and toll-like receptor-2 and -4 expression. Major hepatectomy increased the numbers of activated CD16(bright) blood monocytes and the percentage of activated dendritic cells, although monocyte HLA-DR expression was reduced. These results may represent both dysfunctional antigen presentation and pending anergy, as well as cellular priming of immune effector cells. Better understanding of the alterations in innate immunity induced by hepatectomy may identify strategies to reduce infectious outcomes.
引用
收藏
页码:568 / 581
页数:14
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