Structure and conformation of α-glucan extracted from Agaricus blazei Murill by high-speed shearing homogenization

被引：40

作者：

Zhang, Anqiang ^{[1
]}

Deng, Jiaying ^{[1
]}

Liu, Xiaoqing ^{[1
]}

He, Pengfei ^{[1
]}

He, Liang ^{[2
]}

Zhang, Fuming ^{[3
]}

Linhardt, Robert J. ^{[3
,4
,5
]}

Sun, Peilong ^{[1
]}

机构：

[1] Zhejiang Univ Technol, Dept Food & Technol, Hangzhou 310014, Zhejiang, Peoples R China

[2] Zhejiang Forestry Acad, Key Lab Biol & Chem Utilizat Zhejiang Prov, Hangzhou 310023, Zhejiang, Peoples R China

[3] Rensselaer Polytech Inst, Dept Chem & Biol Engn, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

[4] Rensselaer Polytech Inst, Dept Chem & Chem Biol, Dept Biol Sci, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

[5] Rensselaer Polytech Inst, Dept Biomed Engn, Dept Biol Sci, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 113卷

基金：

中国国家自然科学基金;

关键词：

Agaricus blazei Murill; Polysaccharide; Structure; Chain conformation; High-speed shearing homogenization; ANTIOXIDANT ACTIVITIES; CHAIN CONFORMATION; IN-VITRO; BIOACTIVE POLYSACCHARIDES; IMMUNOMODULATORY ACTIVITY; POLYPORUS-UMBELLATUS; ASSISTED EXTRACTION; MURRILL; OPTIMIZATION; SCLEROTIA;

D O I：

10.1016/j.ijbiomac.2018.02.151

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Agaricus blazei Murill is an edible and medicinal mushroom favored in many countries, by virtue of both its delicious taste and its potential health benefits such as its purported anticancer activity. A neutral alpha-glucan (ABM401) with a carbohydrate content of 96% was purified from the high-speed shearing homogenization extracts of A. Blazei Murill by ethanol precipitation and column chromatography. Methylation analysis along with nuclear magnetic resonance spectroscopy revealed that ABM40-1 was an alpha-(1 -> 4)-D-glucopyranan with 0-6 position occasionally occupied with alpha-Glcp-(1 -> or alpha-Glcp-(1 -> 6)-beta-Glcp-(1 -> side chains. A weight-average molecular weight of 7.34 x 10(6) Da was determined for ABM40-1 and its chain in solution was revealed as a compact sphere by size exclusion chromatography (SEC) coupled with a laser light scattering. This spherical conformation was also further confirmed by Congo red test and using atom force microscopy. These results suggest it would be worthwhile to further study the potential bioactivities of ABM40-1. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：558 / 564

页数：7

共 27 条

[1] Agaritine from Agaricus blazei Murrill induces apoptosis in the leukemic cell line U937 [J].