Global approaches to study Golgi function

Enormous insights into Golgi function have been provided by yeast genetics, biochemical assays and immuno-labeling methods and the emerging picture is of a very complex organelle with multiple levels of regulation. Despite many elegant experimental approaches, it remains unclear what mechanisms transport secretory proteins and lipids through the Golgi, and even the basic structure of the organelle is debated. Recently, new, global approaches such as proteomics and functional genomics have been applied to study the Golgi and its matrix. The data produced reveals great complexity and has potential to help address major unresolved questions concerning Golgi function.