Current approaches in identification and isolation of cancer stem cells

被引：72

作者：

Akbarzadeh, Maryam ^{[1
,2
]}

Maroufi, Nazila Fathi ^{[2
,3
]}

Tazehkand, Abbas Pirpour ^{[2
]}

Akbarzadeh, Moloud ^{[1
,4
]}

Bastani, Sepideh ^{[1
]}

Safdari, Reza ^{[5
]}

Farzane, Ali ^{[5
]}

Fattahi, Amir ^{[6
]}

Nejabati, Hamid Reza ^{[2
,7
]}

Nouri, Mohammad ^{[1
,2
]}

Samadi, Nasser ^{[2
]}

机构：

[1] Tabriz Univ Med Sci, Stem Cell & Regenerat Med Inst, Tabriz, Iran

[2] Tabriz Univ Med Sci, Fac Med, Dept Biochem & Clin Labs, Tabriz 5166614756, Iran

[3] Tabriz Univ Med Sci, Student Res Comm, Tabriz, Iran

[4] Azarbaijan Shahid Madani Univ, Fac Biol Sci, Dept Cellular & Mol Biol, Tabriz, Iran

[5] Univ Tehran Med Sci, Sch Allied Med Sci, Dept Hlth Informat Management, Tehran, Iran

[6] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Reprod Biol, Tabriz, Iran

[7] Tabriz Univ Med Sci, Stem Cell Res Ctr, Tabriz, Iran

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2019年 / 234卷 / 09期

关键词：

aldehyde dehydrogenase; cancer stem cell; side population; spheroid formation assay; surface marker; TUMOR-INITIATING CELLS; ADHESION MOLECULE; PROGENITOR CELLS; CD133; EXPRESSION; PROSTATE-CANCER; POOR-PROGNOSIS; OVARIAN-CANCER; GROWTH-FACTOR; BREAST; SPHEROIDS;

D O I：

10.1002/jcp.28271

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cancer stem cells (CSCs) are tumor cells with initiating ability, self-renewal potential, and intrinsic resistance to conventional therapeutics. Efficient isolation and characterization of CSCs pave the way for more comprehensive knowledge about tumorigenesis, heterogeneity, and chemoresistance. Also a better understanding of CSCs will lead to novel era of both basic and clinical cancer research, reclassification of human tumors, and development of innovative therapeutic strategies. Finding novel diagnostic and effective therapeutic strategies also enhance the success of treatment in cancer patients. There are various methods based on the characteristics of the CSCs to detect and isolate these cells, some of which have recently developed. This review summarized current techniques for effective isolation and characterization of CSCs with a focus on advantages and limitations of each method with clinical applications.

引用

页码：14759 / 14772

页数：14

共 136 条

[1] Isolation, identification, and characterization of cancer stem cells: A review [J].