Inactivation of spoilage yeasts in apple juice by UV-C light and in combination with mild heat

The UV-C resistance of yeasts (Saccharomyces cerevisiae, Saccharomyces bayanus, Zygosaccharomyces bailii, Dekkera anomala and Dekkera bruxellensis) commonly involved in juice spoilage was investigated. Saccharomyces spp. showed the greatest UV resistance and yeast inactivation decreased with absorptivity of the medium. To improve the UV lethal effect on yeasts in juice, UV treatment was combined with heat (UV-H) (45-60 degrees C). The inactivation of S. cerevisiae by UV-H treatments between 52.5 and 57.5 degrees C was greater than use of both technologies individually, suggesting a synergistic effect. Modeling of inactivation data found that a combination of IN-C light with mild temperatures (50-60 degrees C) produced a 5-log10 reduction of S. cerevisiae in clarffiedapple juice with lower UV doses (up to a 89.3% of reduction at 57.5 degrees C) and treatment times (up to 63% of reduction at 52.5 degrees C) than those needed for UV treatments or heat alone. Industrial relevance: This investigation demonstrated the lethal efficacy of the combination of UV-C radiation and heat to improve the inactivation of spoilage yeasts in juice. Combined UV-H treatments synergistically increased yeast inactivation, with this approach being less effective than for bacteria. In any case, UV-H at mild temperatures would be an alternative to heat pasteurization treatments, enabling the inactivation of pathogenic bacteria and reducing the concentration of spoilage microorganisms like yeasts, obtaining a safe and stable product at lower temperatures. (C) 2015 Elsevier Ltd. All rights reserved.