Viral Load Detection Using Dried Blood Spots in a Cohort of HIV-1-Infected Children in Uganda: Correlations with Clinical and Immunological Criteria for Treatment Failure

被引：10

作者：

Costenaro, Paola ^{[1
]}

Lundin, Rebecca ^{[1
]}

Petrara, Maria Raffaella ^{[2
]}

Penazzato, Martina ^{[1
]}

Massavon, William ^{[1
]}

Kizito, Susan ^{[3
]}

Nabachwa, Sandra Monica ^{[3
]}

Musoke, Maria Nannyonga ^{[3
]}

Namisi, Charles ^{[3
]}

Morelli, Erika ^{[1
]}

Bilardi, Davide ^{[1
]}

Mazza, Antonio ^{[4
]}

Zanchetta, Marisa ^{[2
]}

Giaquinto, Carlo ^{[1
]}

De Rossi, Anita ^{[2
,5
]}

机构：

[1] Univ Padua, Dept Pediat, Padua, Italy

[2] Univ Padua, Dept Surg Oncol & Gastroenterol, Sect Oncol & Immunol, AIDS Reference Ctr, Padua, Italy

[3] St Raphael St Francis Nsambya Hosp, Nsambya Home Care Dept, Kampala, Uganda

[4] Assoc Casa Accoglienza Vita Padre Angelo, Trento, Italy

[5] IRCCS, IOV, Padua, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2014年 / 52卷 / 07期

关键词：

DRUG-RESISTANCE; HIV; SPECIMENS; PLASMA;

D O I：

10.1128/JCM.00961-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Correlations between clinical/immunological treatment failure and viral load (VL) detected by dried blood spot (DBS) sampling were explored in HIV-1-infected children in Uganda. Of 104 children on combined antiretroviral treatment (cART), 12.5% experienced clinical and/or immunological failure, while 28.8%, 44.2%, and 26.9% had VLs of <1,000, 1,000 to 5,000, and >5,000 copies/ml, respectively. Clinical/immunological failure poorly predicted virological failure.

引用

收藏

页码：2665 / 2667

页数：3

相关论文

共 15 条

[1] Comparison of HIV-1 RNA Measurements Obtained by Using Plasma and Dried Blood Spots in the Automated Abbott Real-Time Viral Load Assay [J].

Arredondo, Miguel ;

Garrido, Carolina ;

Parkin, Neil ;

Zahonero, Natalia ;

Bertagnolio, Silvia ;

Soriano, Vincent ;

de Mendoza, Carmen .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (03) :569-572

[2]

Bertagnolio S, 2010, AIDS REV, V12, P195

[3] Development of Antiretroviral Resistance in Children With HIV in Low- and Middle-Income Countries [J].

Fitzgerald, Felicity ;

Penazzato, Martina ;

Gibb, Diana .

JOURNAL OF INFECTIOUS DISEASES, 2013, 207 :S85-S92

[4] Concurrent measures of total and integrated HIV DNA monitor reservoirs and ongoing replication in eradication trials [J].

Mexas, Angela M. ;

Graf, Erin H. ;

Pace, Matthew J. ;

Yu, Jianqing J. ;

Papasavvas, Emmanouil ;

Azzoni, Livio ;

Busch, Michael P. ;

Di Mascio, Michele ;

Foulkes, Andrea S. ;

Migueles, Stephen A. ;

Montaner, Luis J. ;

O'Doherty, Una .

AIDS, 2012, 26 (18) :2295-2306

[5] Evaluation of Different RNA Extraction Methods and Storage Conditions of Dried Plasma or Blood Spots for Human Immunodeficiency Virus Type 1 RNA Quantification and PCR Amplification for Drug Resistance Testing [J].

Monleau, Marjorie ;

Montavon, Celine ;

Laurent, Christian ;

Segondy, Michel ;

Montes, Brigitte ;

Delaporte, Eric ;

Boillot, Francois ;

Peeters, Martine .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (04) :1107-1118

[6] Evaluation of In-house Genotyping Assay Performance Using Dried Blood Spot Specimens in the Global World Health Organization Laboratory Network [J].

Parkin, Neil ;

de Mendoza, Carmen ;

Schuurman, Rob ;

Jennings, Cheryl ;

Bremer, James ;

Jordan, Michael R. ;

Bertagnolio, Silvia .

CLINICAL INFECTIOUS DISEASES, 2012, 54 :S273-S279

[7]

Petrara MR, 2014, J INFECT DIS

[8] Failure of immunologic criteria to appropriately identify antiretroviral treatment failure in Uganda [J].

Reynolds, Steven J. ;

Nakigozi, Gertrude ;

Newell, Kevin ;

Ndyanabo, Anthony ;

Galiwongo, Ronald ;

Roaz, Iga ;

Quinn, Thomas C. ;

Gray, Ron ;

Wawer, Maria ;

Serwadda, David .

AIDS, 2009, 23 (06) :697-700

[9] Dried Blood Spot Specimens Are a Suitable Alternative Sample Type for HIV-1 Viral Load Measurement and Drug Resistance Genotyping in Patients Receiving First-Line Antiretroviral Therapy [J].

Rottinghaus, Erin K. ;

Ugbena, Richard ;

Diallo, Karidia ;

Bassey, Orji ;

Azeez, Aderemi ;

DeVos, Joshua ;

Zhang, Guoqing ;

Aberle-Grasse, John ;

Nkengasong, John ;

Yang, Chunfu .

CLINICAL INFECTIOUS DISEASES, 2012, 54 (08) :1187-1195

[10] Systematic Review of the Use of Dried Blood Spots for Monitoring HIV Viral Load and for Early Infant Diagnosis [J].

Smit, Pieter W. ;

Sollis, Kimberly A. ;

Fiscus, Susan ;

Ford, Nathan ;

Vitoria, Marco ;

Essajee, Shaffiq ;

Barnett, David ;

Cheng, Ben ;

Crowe, Suzanne M. ;

Denny, Thomas ;

Landay, Alan ;

Stevens, Wendy ;

Habiyambere, Vincent ;

Perriens, Joseph H. ;

Peeling, Rosanna W. .

PLOS ONE, 2014, 9 (03)