Supercritical carbon dioxide extraction of antioxidant rich fraction from Phormidium valderianum: Optimization of experimental process parameters

被引:28
作者
Chatterjee, Dipan [1 ]
Bhattacharjee, Paramita [1 ]
机构
[1] Jadavpur Univ, Dept Food Technol & Biochem Engn, Kolkata 700032, India
来源
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | 2014年 / 3卷
关键词
Phormidium valderianum; Supercritical carbon dioxide extraction; Antioxidant-rich fraction; Minimum Anatoxin-a content; FATTY-ACID-COMPOSITION; FLUID EXTRACTION; C-PHYCOCYANIN; ANATOXIN-A; SPIRULINA; OIL;
D O I
10.1016/j.algal.2013.11.014
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Extraction of antioxidant-rich fractions from lyophilized algal biomass of Phormidium valderianum was carried out by green technology of supercritical carbon dioxide (SC-CO2) extraction. Optimization of extraction parameters (temperature and pressure) was carried out to obtain an extract that has the best combination of phytochemical properties (phenolic content, carotenoid content, reducing power and antioxidant activity) with minimum toxin content (Anatoxin-a). The algal extract obtained by SC-CO2 from 10 g biomass at 50 degrees C, 500 bar after 1.5 h extracting time at a flow rate of 2 l min(-1) of gaseous CO2 had maximum phytochemical properties with similar to 93% reduction of Anatoxin-a content. This investigation provides an alternative method for obtaining natural antioxidants from algae for food and pharmaceutical applications. (C) 2013 Elsevier B. V. All rights reserved.
引用
收藏
页码:49 / 54
页数:6
相关论文
共 27 条
[1]   Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC [J].
Aiyegoro, Olayinka A. ;
Okoh, Anthony I. .
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2010, 10
[2]   Supercritical fluid extraction of oil from microalga Spirulina(arthrospira) platensis [J].
Andrich, G. ;
Zinnai, A. ;
Nesti, U. ;
Venturi, F. ;
Fiorentini, R. .
ACTA ALIMENTARIA, 2006, 35 (02) :195-203
[3]   Supercritical fluid extraction of bioactive lipids from a the microalga Nannochloropsis sp. [J].
Andrich, G ;
Nesti, U ;
Venturi, F ;
Zinnai, A ;
Fiorentini, R .
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 2005, 107 (06) :381-386
[4]  
[Anonymous], 1986, Nippon Shokuin Kogyo Gakkaishi, DOI DOI 10.3136/NSKKK1962.35.11_771
[5]  
Association of Official Analytical Chemists (AOAC), 2006, Official Methods of Analysis AOAC International
[6]  
Benzie IFF, 1999, METHOD ENZYMOL, V299, P15
[7]   C-Phycocyanin:: A potent peroxyl radical scavenger in vivo and in vitro [J].
Bhat, VB ;
Madyastha, KM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 275 (01) :20-25
[8]   Supercritical Carbon Dioxide Extraction of Eugenol from Clove Buds [J].
Chatterjee, Dipan ;
Bhattacharjee, Paramita .
FOOD AND BIOPROCESS TECHNOLOGY, 2013, 6 (10) :2587-2599
[9]   FATTY-ACID COMPOSITION OF SPIRULINA AND SPIRULINA-LIKE CYANOBACTERIA IN RELATION TO THEIR CHEMOTAXONOMY [J].
COHEN, Z ;
VONSHAK, A .
PHYTOCHEMISTRY, 1991, 30 (01) :205-206
[10]   Comparison of essential oils of clove buds extracted with supercritical carbon dioxide and other three traditional extraction methods [J].
Guan Wenqiang ;
Li Shufen ;
Yan Ruixiang ;
Tang Shaokun ;
Quan Can .
FOOD CHEMISTRY, 2007, 101 (04) :1558-1564