lncRNA FOXD2-AS1 affects trophoblast cell proliferation, invasion and migration through targeting miRNA

被引：19

作者：

Zhang, Yulei ^{[1
]}

Chen, Xiaoqin ^{[1
]}

机构：

[1] Huaian First Peoples Hosp, Obstet Dept, Huaian 223300, Jiangsu, Peoples R China

来源：

ZYGOTE | 2020年 / 28卷 / 02期

关键词：

Invasion; lncRNA FOXD2-AS1; Migration; miR-3127; Proliferation; PREECLAMPSIA; APOPTOSIS; PATHWAY;

D O I：

10.1017/S0967199419000807

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The abnormal expression of lncRNAs and miRNAs has been found in the placentas of patients with preeclampsia (PE). Therefore, we determined the role of lncRNA FOXD2-AS1/miR-3127 in trophoblast cells. The expression of lncRNA FOXD2-AS1 was detected by qRT-PCR. The proliferation, migration and invasion ability of trophoblast cells were evaluated using CCK-8, wound healing and transwell assays. The target gene of lncRNA FOXD2-AS1 was determined by StarBase and luciferase reporter assays. Western blotting was used to analyze the expression of matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP9). The results showed that FOXD2-AS1 affected trophoblast cell viabilityin vitro, while the expression of miR-3127 was decreased. FOXD2-AS1 silencing decreased the promotion effects on trophoblast cell induced by miR-3127 inhibition. In addition, FOXD2-AS1 and miR-3127 presented the same effect on MMP2 and MMP9 levels. lncRNA FOXD2-AS1 modulated trophoblast cell proliferation, invasion and migration through downregulating miR-3127 expression. Therefore, lncRNA FOXD2-AS1 could act as a latent therapeutic marker in preeclampsia.

引用

页码：131 / 138

页数：8

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