Large-scale analysis of glycoproteins by LC-MS method

被引：3
|
作者：
Kaji, Hiroyuki [1 ]
Isobe, Toshiaki [1 ]
机构：
[1] Tokyo Metropolitan Univ, Grad Sch Sci & Engn, Dept Chem, Tokyo 1920397, Japan
来源：
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY | 2006年 / 18卷 / 103期
关键词：
proteomics; glycoprotein; mass spectrometry; lectin; LC-MS;
D O I：
10.4052/tigg.18.313
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This review presents a strategy and applications of the large-scale identification of N-glycosylated proteins and their glycosylated sites. The technology is based on the affinity capture of glycopeptides with lectins, the enzyme-mediated stable isotope-labeling of N-glycosylated sites, and the liquid chromatography/mass spectrometry (LC-MS) shotgun analysis.
引用
收藏
页码：313 / 322
页数：10
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