A comparative study of indirect competitive ELISA and HPLC for fumonisin detection in corn of the state of Parana, Brazil

被引：0
作者：
Ono, EYS
Kawamura, O
Ono, MA
Ueno, Y
Hirooka, EY
机构：
[1] State Univ Londrina, Dept Biochem CCE, BR-86051990 Londrina, Parana, Brazil
[2] Sci Univ Tokyo, Fac Pharmaceut Sci, Tokyo 1620826, Japan
[3] State Univ Londrina, Dept Pathol Sci CCB, BR-86051990 Londrina, Parana, Brazil
[4] State Univ Londrina, Dept Food & Drug Technol CCA, BR-86051990 Londrina, Parana, Brazil
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2000年 / 12卷 / 01期
关键词：
fumonisins; ELISA; HPLC; corn;
D O I：
10.1080/09540100099580
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The performance of a monoclonal antibody (MAb)-based indirect competitive ELISA (IC-ELISA) for fumonisin detection in corn was valuated using 150 post-harvest corn samples from the April 1995 and March-April 1996 crop, in the State of parana, Brazil. Compared to high performance liquid chromatography (HPLC), the correlation coefficient (r) of the results from the two methods was 0.94. The IC-ELISA detected total fumonisin levels of 1.1 to 1.5-fold higher than HPLC in 85 samples (56.6%). The concentration range of 1.0 to 5.0 mu g g(-1) was observed in 26% of samples analysed by ELISA and in 29% by HPLC. In addition, four samples (2.7%) showed an ELISA/HPLC ratio of 0.7 to 0.99 and seven samples (4.7%) a 1.0 ratio. In 29 (13.3%) samples, IC-ELISA detected 1.6 to 2.0-fold higher values than HPLC. These data showed high correlation (r = 0.91) of IC-ELISA and HPLC for fumonisin concentration in corn contaminated with less than or equal to 10 mu g g(-1), with a detection limit of 93 ng g(-1). Matrix interference was minimized by diluting the sample before the ELISA assay. MAb-based IC-ELISA may be effectively applied for fumonisin screening in corn, with advantages of simplicity, ease of sample preparation and sensitivity.
引用
收藏
页码：5 / 14
页数：10
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