Influence of Chitosan Derivatization on Its Physicochemical Characteristics and Its Use as Enzyme Support

被引:17
作者
Urrutia, Paulina [1 ]
Bernal, Claudia [1 ,2 ]
Escobar, Sindy [2 ]
Santa, Cristiam [2 ]
Mesa, Monica [2 ]
Wilson, Lorena [1 ]
Illanes, Andres [1 ]
机构
[1] Pontificia Univ Catolica Valparaiso, Escuela Ingn Bioquim, Valparaiso 2147, Chile
[2] Univ Antioquia, Inst Quim, Grp Ciencia Mat, Medellin, Colombia
关键词
Biopolymers and renewable polymers; polysaccharides; crosslinking; proteins; MULTIPOINT COVALENT IMMOBILIZATION; BETA-GALACTOSIDASE; BACILLUS-CIRCULANS; THERMAL-STABILITY; CROSS-LINKING; IMPROVEMENT; AGAROSE; LACTOSE; LIPASE; OLIGOSACCHARIDES;
D O I
10.1002/app.40171
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Chitosan was derivatized by two methodologies for analyzing their effect on chitosan physicochemical characteristics and its applicability as carrier for Bacillus circulans -galactosidase immobilization. Glutaraldehyde (GA) and epichlorohydrin (EPI) were used for crosslinking and activation of chitosan, producing the corresponding supports (C-GA and C-EPI-EPI) after a one-step and a two-step process, respectively. The spherical shape and mean diameter of chitosan particles was not significantly affected by polymer derivatization, while Fourier transform infrared analysis showed that in both cases, chitosan polymer was chemically modified. TGA analysis indicated that C-EPI-EPI was the most thermally stable. The high degree of activation of C-EPI-EPI (586 mol of aldehydes/g) resulted in the highest loss of activity during immobilization; hence a support with 100 mol of aldehydes/g was produced (C-EPI-EPI100). The highest expressed activity (89.3 IU/g) was obtained with the enzyme immobilized in C-GA, while the biocatalyst with highest thermal stability at 60 degrees C was obtained with C-EPI-EPI100 (half-life was 84-fold higher than the one of the soluble enzyme). The best compromise between biocatalyst expressed activity and thermal stability corresponded to -galactosidase immobilized in C-EPI-EPI100. According to this study, chitosan derivatized with EPI is a thermally stable carrier appropriate for producing highly stable immobilized B. circulans -galactosidase. (c) 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40171.
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页数:10
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