Cytotoxic response in human lung epithelial cells and ion characteristics of urban-air particles from Torino, a northern Italian city

被引:52
作者
Alessandria, Luca [1 ]
Schiliro, Tiziana [1 ]
Degan, Raffaella [1 ]
Traversi, Deborah [1 ]
Gilli, Giorgio [1 ]
机构
[1] Univ Torino, Dept Publ Hlth & Pediat, I-10126 Turin, Italy
关键词
PARTICULATE-MATTER PM2.5; OXIDATIVE STRESS; IN-VITRO; ALVEOLAR MACROPHAGES; SOURCE APPORTIONMENT; PERSONAL EXPOSURE; ORGANIC EXTRACTS; POLLUTION; PM10; FINE;
D O I
10.1007/s11356-013-2468-1
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Recently, much attention has been devoted to urban air pollution because epidemiological studies have reported health impacts related to particulate matter (PM). PM10 and PM2.5 were collected during different seasons in Torino, a northern Italian city, and were characterised by inorganic chemical species (secondary particulates and bio-available iron). The biological effects of aqueous and organic solvent PM extracts on human epithelial lung A549 were evaluated, and the effects on cell proliferation and lactate dehydrogenase (LDH) release were assayed. The average PM10 concentration during the sampling period was 47.9 +/- 18.0 mu g/m(3); the secondary particles accounted for 49 % +/- 9 % of the PM10 total mass, and the bio-available iron concentration was 0.067 +/- 0.045 mu g/m(3). The PM2.5/PM10 ratio in Torino ranged from 0.47 to 0.90 and was higher in cold months than in warm months. The PM10 and PM2.5 extracts inhibited cell proliferation and induced LDH release in a dose-dependent manner with a seasonal trend. The PM10 extract had a stronger effect on LDH release, whereas the PM2.5 extract more strongly inhibited cell proliferation. No significant differences were observed in the effects induced by the two extracts, and no significant correlations were found between the biological effects and the PM components evaluated in this study, thus emphasising the importance of the entire mixture in inducing a cytotoxic response.
引用
收藏
页码:5554 / 5564
页数:11
相关论文
共 75 条
[1]   Biologic effects induced in vitro by PM10 from three different zones of Mexico City [J].
Alfaro-Moreno, E ;
Martínez, L ;
García-Cuellar, C ;
Bonner, JC ;
Murray, JC ;
Rosas, I ;
Rosales, SPD ;
Osornio-Vargas, AR .
ENVIRONMENTAL HEALTH PERSPECTIVES, 2002, 110 (07) :715-720
[2]  
[Anonymous], 1967, Methods Enzymol., DOI DOI 10.1016/0076-6879(67)10078-5
[3]  
[Anonymous], 2000, RES REP HLTH EFF I
[4]  
[Anonymous], THORAX
[5]  
[Anonymous], HLTH IMP PM10 OZ 13
[6]  
[Anonymous], JOINTWHO CONV TASK F
[7]  
[Anonymous], EPA600P95001A
[8]  
[Anonymous], ENV HLTH
[9]   PM2.5 assessment in 21 European study centers of ECRHS II:: Method and first winter results [J].
Arx, MEHV ;
Fellmann, TG ;
Oglesby, L ;
Liebrich, UA ;
Gíslason, T ;
Heinrich, J ;
Jarvis, D ;
Luczynska, C ;
Manzanera, AJ ;
Modig, L ;
Norbäck, D ;
Pfeifer, A ;
Poli, A ;
Ponzio, M ;
Soon, A ;
Vermeire, P ;
Künzli, N .
JOURNAL OF THE AIR & WASTE MANAGEMENT ASSOCIATION, 2003, 53 (05) :617-628
[10]   Biological effects of atmospheric particles on human bronchial epithelial cells. Comparison with diesel exhaust particles [J].
Baulig, A ;
Sourdeval, M ;
Meyer, M ;
Marano, F ;
Baeza-Squiban, A .
TOXICOLOGY IN VITRO, 2003, 17 (5-6) :567-573