Impact of membrane protein-lipid interactions on formation of bilayer lipid membranes on SAM-modified gold electrode

被引：6

作者：

Kato, Masaru ^{[1
,2
,3
]}

Masuda, Yuya ^{[2
]}

Yoshida, Narumi ^{[2
]}

Tosha, Takehiko ^{[4
]}

Shiro, Yoshitsugu ^{[5
]}

Yagi, Ichizo ^{[1
,2
,3
]}

机构：

[1] Hokkaido Univ, Fac Environm Earth Sci, Kita Ku, N10W5, Sapporo, Hokkaido 0600810, Japan

[2] Hokkaido Univ, Grad Sch Environm Sci, Kita Ku, N10W5, Sapporo, Hokkaido 0600810, Japan

[3] Natl Inst Mat Sci NIMS, Global Res Ctr Environm & Energy Based Nanomat Sc, Tsukuba, Ibaraki 3050044, Japan

[4] RIKEN SPring8 Ctr, Sayo, Hyogo 6795148, Japan

[5] Univ Hyogo, Grad Sch Life Sci, Kakogawa, Hyogo 6781297, Japan

来源：

ELECTROCHIMICA ACTA | 2021年 / 373卷

关键词：

Membrane protein; Surface-enhanced infrared absorption; spectroscopy; Nitric oxide reductase; Protein-tethered bilayer lipid membrane; Protein film electrochemistry; SPECTROSCOPY;

D O I：

10.1016/j.electacta.2021.137888

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

In protein film electrochemistry for redox-active membrane proteins, the reconstitution of protein tethered bilayer lipid membranes (ptBLMs) can maximize their functionality and stability at electrolyte/electrode interfaces. To understand the impact of protein-lipid interactions on the ptBLM formation, we tracked the reconstitution process of a lipid bilayer in the presence or the absence of a trans membrane enzyme of cytochrome c-dependent nitric oxide reductase (cNOR) on a mixed self-assembled monolayer (SAM)/Au electrode. Time-resolved surface-enhanced infrared absorption (SEIRA) spectroscopy and electrochemical measurements revealed that the protein-lipid interaction affected the formation kinetics for the phospholipid bilayer and the electrocatalytic stability of the cNOR-modified electrode but not the fluidity of the phospholipid bilayer. (c) 2021 Elsevier Ltd. All rights reserved.

引用

页数：7

共 50 条

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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (04) : 1542 - 1546
[2] THEORY OF PROTEIN-LIPID AND PROTEIN-PROTEIN INTERACTIONS IN BILAYER MEMBRANES
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