Human high-density lipoprotein microtranscriptome is unique and suggests an extended role in lipid metabolism

被引：8

作者：

Desgagne, Veronique ^{[1
,2
]}

Guerin, Renee ^{[1
,3
]}

Guay, Simon-Pierre ^{[1
,2
,4
,5
]}

Boyer, Marjorie ^{[6
,7
]}

Hutchins, Elizabeth ^{[8
]}

Picard, Samuel ^{[9
]}

Marechal, Alexandre ^{[9
]}

Corbin, Francois ^{[1
]}

Van Keuren-Jensen, Kendall ^{[8
]}

Arsenault, Benoit J. ^{[6
,7
]}

Bouchard, Luigi ^{[1
,2
,3
]}

机构：

[1] Univ Sherbrooke, Dept Biochem, Sherbrooke, PQ J1H 5N4, Canada

[2] Hop Chicoutimi, ECOGENE 21 Biocluster, CIUSSS Saguenay Lac St Jean, Saguenay, PQ G7H 5H6, Canada

[3] Hop Chicoutimi, Dept Lab Med, CIUSSS Saguenay Lac St Jean, Saguenay, PQ G7H 5H6, Canada

[4] Univ Sherbrooke, Dept Med, PFMS, Sherbrooke, PQ G7H 2B1, Canada

[5] McGill Univ, Dept Med Genet, MUHC, Montreal, PQ H4A 3J1, Canada

[6] IUCPQ, Ctr Rech, Quebec City, PQ G1V 4G5, Canada

[7] Univ Laval, Dept Med, Fac Med, Quebec City, PQ G1V 0A6, Canada

[8] Translat Genom Res Inst TGen, Neurogen Div, Phoenix, AZ 85004 USA

[9] Univ Sherbrooke, Dept Biol, Sherbrooke, PQ J1K 2R1, Canada

来源：

EPIGENOMICS | 2019年 / 11卷 / 08期

基金：

加拿大自然科学与工程研究理事会;

关键词：

cardiovascular risk; functionality; high-density lipoprotein; human; lipid; microRNA; microtranscriptome; next-generation sequencing; plasma; sex; CHOLESTEROL EFFLUX CAPACITY; DIETARY TRANS FAT; CARDIOVASCULAR-DISEASE; HDL; MICRORNAS; PLASMA; RECEPTOR; MARKERS; CONSUMPTION; BIOMARKERS;

D O I：

10.2217/epi-2018-0161

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To comprehensively characterize the high-density lipoproteins (HDLs) microtranscriptome and to assess whether it is distinct from that of plasma and different between women and men. Methods: RNA was extracted from ultracentrifugation-purified HDLs and plasma from 17 healthy women and men couples, and libraries were sequenced on a HiSeq2500 platform. Results: On average, 310 +/- 64 and 355 +/- 31 miRNAs were detected (>= 1 read per million) in HDLs and plasma, respectively. A total of 62 and 134 miRNAs were over-represented (e.g., miR-150-5p; fold change = 7.52; p(adj) = 5.41 x 10(-111)) and under-represented (e.g., miR-22-3p; fold change = -5.28; p(adj) = 2.11 x 10(-154)) in HDLs compared with plasma. These miRNAs were enriched in lipid metabolism and cellular processes-related pathways. Conclusion: HDLs exhibit a sex-independent miRNA profile distinct from that of plasma. These miRNAs may contribute to the HDLs' physiology.

引用

页码：917 / 934

页数：18

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