Trematode Fluke Procerovum varium as Cause of Ocular Inflammation in Children, South India

被引：25

作者：

Arya, Lalan Kumar ^{[1
]}

Rathinam, Sivakumar R. ^{[2
]}

Lalitha, Prajna ^{[1
]}

Kim, Usha R. ^{[2
]}

Ghatani, Sudeep ^{[3
]}

Tandon, Veena ^{[3
]}

机构：

[1] Aravind Med Res Fdn, Madurai, Tamil Nadu, India

[2] Aravind Eye Hosp & Postgrad Inst Ophthalmol, Madurai, Tamil Nadu, India

[3] NE Hill Univ, Shillong 793014, Meghalaya, India

来源：

EMERGING INFECTIOUS DISEASES | 2016年 / 22卷 / 02期

关键词：

POLYMERASE-CHAIN-REACTION; PCR-BASED DETERMINATION; FISH-BORNE TREMATODES; ANTERIOR UVEITIS; QUANTITATIVE DETECTION; HUMAN-EYE; PHILOPHTHALMUS; CONJUNCTIVA; INFECTIONS; THAILAND;

D O I：

10.3201/eid2202.150051

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Trematodes are recognized as a group of emerging parasites in tropical countries. We identified a trematode as a cause of ocular granulomas that developed in children who bathed in ponds or rivers in South India. DNA was isolated from patients' surgically excised granulomas and from the trematode cercariae (larvae) released by the snail Melanoides tuberculate in water in which the children bathed. Real-time and conventional PCRs were performed that targeted ribosomal DNA regions spanning the internal transcribed spacer 2 and 28S sequences of this trematode. The PCR-amplified products were subjected to bidirectional sequencing. Analysis of sequences for the granuloma samples and the trematode cercariae showed maximum sequence similarity with Procerovum varium (family Heterophyidae). Our results confirmed the etiology of the ocular infection, implicating snail vectors as environmental risk factors for ocular parasitosis.

引用

页码：192 / 200

页数：9

共 38 条

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[2]

[Anonymous], TAMIL NADU OPHTHALMI

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