Luminescent CdSe-ZnS quantum dots as selective Cu2+ probe

被引：184

作者：

Xie, HY ^{[1
]}

Liang, JG ^{[1
]}

Zhang, ZL ^{[1
]}

Liu, Y ^{[1
]}

He, ZK ^{[1
]}

Pang, DW ^{[1
]}

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2004年 / 60卷 / 11期

基金：

中国国家自然科学基金;

关键词：

quantum dots; probe; luminescent; mechanism;

D O I：

10.1016/j.saa.2003.12.039

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Luminescent CdSe-ZnS quantum dots (QDs) were modified with bovine serum albumin (BSA) and used as selective copper ion probe. The fluorescence of the water-soluble QDs can be quenched only by Cu2+ and Fe3+ in physiological buffer solution. Approximate concentrations of other physiologically important cations, such as Zn2+, Na+ and K+ etc. have no effect on the fluorescence. Adding F- to form the colorless complex FeF63- can eliminate the interference of Fe3+. The detection limit Of Cu2+ ions was 10 nM. The results can be explained in terms of strong binding of Cu2+ onto the surface of CdSe resulting in a chemical displacement of Cd2+ ions and the formation of CuSe on the surface of the QDs. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：2527 / 2530

页数：4

共 17 条

[1] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[2]

CHAN W, 2001, THESIS INDIANA U

[3] Luminescent quantum dots for multiplexed biological detection and imaging [J].

Chan, WCW ;

Maxwell, DJ ;

Gao, XH ;

Bailey, RE ;

Han, MY ;

Nie, SM .

CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (01) :40-46

[4] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[5] Luminescent CdS quantum dots as selective ion probes [J].

Chen, YF ;

Rosenzweig, Z .

ANALYTICAL CHEMISTRY, 2002, 74 (19) :5132-5138

[6] Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules [J].

Han, MY ;

Gao, XH ;

Su, JZ ;

Nie, S .

NATURE BIOTECHNOLOGY, 2001, 19 (07) :631-635

[7] Amino terminal Cu(II)- and Ni(II)-binding (ATCUN) motif of proteins and peptides: Metal binding, DNA cleavage, and other properties [J].

Harford, C ;

Sarkar, B .

ACCOUNTS OF CHEMICAL RESEARCH, 1997, 30 (03) :123-130

[8]

HASSELBARTH A, 1993, J PHYS CHEM-US, V97, P5333

[9]

HE ZK, Patent No. 03118483

[10] Optical and photochemical properties of nonstoichiometric cadmium sulfide nanoparticles: Surface modification with copper(II) ions [J].

Isarov, AV ;

Chrysochoos, J .

LANGMUIR, 1997, 13 (12) :3142-3149

← 1 2 →