PUVA-induced apoptosis involves mitochondrial dysfunction caused by the opening of the permeability transition pore

被引：55

作者：

Canton, M

Caffieri, S

Dall'Acqua, F

Di Lisa, F

机构：

[1] Univ Padua, Dipartimento Chim Biol, I-35121 Padua, Italy

[2] Univ Padua, Dipartimento Sci Farmaceut, Padua, Italy

[3] Univ Padua, CNR, Ist Neurosci, Sez Biomembrane, Padua, Italy

来源：

FEBS LETTERS | 2002年 / 522卷 / 1-3期

关键词：

psoralen; apoptosis; permeability transition; mitochondria; cyclosporin A;

D O I：

10.1016/S0014-5793(02)02926-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanism of cell death was investigated in Jurkat cells exposed to the combination of psoralen and UVA irradiation (PUVA). Apoptosis was by far prevailing over necrosis and involved mitochondrial dysfunction. The collapse of mitochondrial membrane potential, appears to be caused by the opening of the mitochondrial permeability transition pore since its inhibitor, cyclosporin A, prevented mitochondrial dysfunction and largely attenuated apoptosis. Apoptosis also occurred in cells treated with the photoproducts generated by irradiating psoralen in vitro with an oxygen-dependent process. Thus, the involvement of reactive oxygen species in the onset of PUVA-induced apoptosis appears mostly related to psoralen photooxidation. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：168 / 172

页数：5