Gramicidin D enhances the antibacterial activity of fluoride

被引:11
|
作者
Nelson, James W. [1 ]
Zhou, Zhiyuan [2 ]
Breaker, Ronald R. [2 ,3 ,4 ]
机构
[1] Yale Univ, Dept Chem, New Haven, CT 06520 USA
[2] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
[3] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
[4] Howard Hughes Med Inst, Chevvy Chase, MD 20815 USA
基金
美国国家卫生研究院;
关键词
Antibiotic; Bacillus subtilis; Fluoride reporter; Riboswitch; Polymyxin B; CHANNEL; PERMEABILITY; ENVIRONMENT; TOXICITY; OUTER;
D O I
10.1016/j.bmcl.2014.03.061
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Fluoride is a toxic anion found in many natural environments. One of the major bacterial defenses against fluoride is the cell envelope, which limits passage of the membrane-impermeant fluoride anion. Accordingly, compounds that enhance the permeability of bacterial membranes to fluoride should also enhance fluoride toxicity. In this study, we demonstrate that the pore-forming antibiotic gramicidin D increases fluoride uptake in Bacillus subtilis and that the antibacterial activity of this compound is potentiated by fluoride. Polymyxin B, another membrane-targeting antibiotic with a different mechanism of action, shows no such improvement. These results, along with previous findings, indicate that certain compounds that destabilize bacterial cell envelopes can enhance the toxicity of fluoride. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2969 / 2971
页数:3
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