Size effect on transfection and cytotoxicity of nanoscale plasmid DNA/polyethyleneimine complexes for aerosol gene delivery

被引：1

作者：

Byeon, Jeong Hoon ^{[1
]}

Kim, Jang-Woo ^{[2
]}

机构：

[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[2] Hoseo Univ, Dept Digital Display Engn, Asan 336795, South Korea

来源：

APPLIED PHYSICS LETTERS | 2014年 / 104卷 / 05期

关键词：

GAS-PHASE SYNTHESIS; IN-VITRO; MODIFIED POLYETHYLENIMINES; PDNA/PEI COMPLEXES; JET NEBULIZATION; THERAPY; LUNG; EXPRESSION; DNA; NANOPARTICLES;

D O I：

10.1063/1.4864268

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Nanoscale plasmid DNA (pDNA)/polyethyleneimine (PEI) complexes were fabricated in the aerosol state using a nebulization system consisting of a collison atomizer and a cool-walled diffusion dryer. The aerosol fabricated nanoscale complexes were collected and employed to determine fundamental properties of the complexes, such as size, structure, surface charge, and in vitro gene transfection efficiency and cytotoxicity. The results showed that mass ratio between pDNA and PEI should be optimized to enhance gene transfection efficiency without a significant loss of cell viability. These findings may support practical advancements in the field of nonviral gene delivery. (C) 2014 AIP Publishing LLC.

引用

收藏

页数：4

相关论文

共 23 条

[1] Continuous gas-phase synthesis of graphene nanoflakes hybridized by gold nanocrystals for efficient water purification and gene transfection [J].

Byeon, Jeong Hoon ;

Kim, Young-Woo .

CHEMICAL ENGINEERING JOURNAL, 2013, 229 :540-546

[2] Hybrid gas-phase synthesis of nanoscale Fe-SiO2 core-shell agglomerates for efficient transfection into cells and use in magnetic cell patterning [J].

Byeon, Jeong Hoon ;

Kim, Young-Woo .

RSC ADVANCES, 2013, 3 (33) :13685-13689

[3] Monodisperse Poly(lactide-co-glycolic acid)-Based Nanocarriers for Gene Transfection [J].

Byeon, Jeong Hoon ;

Kim, Hee-Kwon ;

Roberts, Jeffrey T. .

MACROMOLECULAR RAPID COMMUNICATIONS, 2012, 33 (21) :1840-1844

[4] Aerosol Based Fabrication of Thiol-Capped Gold Nanoparticles and Their Application for Gene Transfection [J].

Byeon, Jeong Hoon ;

Roberts, Jeffrey T. .

CHEMISTRY OF MATERIALS, 2012, 24 (18) :3544-3549

[5] Biophysical characterization of PEI/DNA complexes [J].

Choosakoonkriang, S ;

Lobo, BA ;

Koe, GS ;

Koe, JG ;

Middaugh, CR .

JOURNAL OF PHARMACEUTICAL SCIENCES, 2003, 92 (08) :1710-1722

[6] Modified polyethylenimines as non viral gene delivery systems for aerosol therapy:: effects of nebulization on cellular uptake and transfection efficiency [J].

Dailey, LA ;

Kleemann, E ;

Merdan, T ;

Petersen, H ;

Schmehl, T ;

Gessler, T ;

Hänze, J ;

Seeger, W ;

Kissel, T .

JOURNAL OF CONTROLLED RELEASE, 2004, 100 (03) :425-436

[7] Enhanced lung gene expression after aerosol delivery of concentrated pDNA/PEI complexes [J].

Davies, Lee A. ;

McLachlan, Gerry ;

Sumner-Jones, Stephanie G. ;

Ferguson, David ;

Baker, Alison ;

Tennant, Peter ;

Gordon, Catherine ;

Vrettou, Christina ;

Baker, Eilidh ;

Zhu, Jie ;

Alton, Eric W. F. W. ;

Collie, David D. S. ;

Porteous, David J. ;

Hyde, Stephen C. ;

Gill, Deborah R. .

MOLECULAR THERAPY, 2008, 16 (07) :1283-1290

[8] The use of CpG-free plasmids to mediate persistent gene expression following repeated aerosol delivery of pDNA/PEI complexes [J].

Davies, Lee A. ;

Hyde, Stephen C. ;

Nunez-Alonso, Graciela ;

Bazzani, Reto P. ;

Harding-Smith, Rebekka ;

Pringle, Ian A. ;

Lawton, Anna E. ;

Abdullah, Syahril ;

Roberts, Thomas C. ;

McCormick, Dominique ;

Sumner-Jones, Stephanie G. ;

Gill, Deborah R. .

BIOMATERIALS, 2012, 33 (22) :5618-5627

[9] Targeting of the β2-adrenoceptor increases nonviral gene delivery to pulmonary epithelial cells in vitro and lungs in vivo [J].

Elfinger, Markus ;

Geiger, Johannes ;

Hasenpusch, Guenther ;

Uezguen, Senta ;

Sieverling, Nathalie ;

Aneja, Manish K. ;

Maucksch, Christof ;

Rudolph, Carsten .

JOURNAL OF CONTROLLED RELEASE, 2009, 135 (03) :234-241

[10] Protective copolymers for nonviral gene vectors: synthesis, vector characterization and application in gene delivery [J].

Finsinger, D ;

Remy, JS ;

Erbacher, P ;

Koch, C ;

Plank, C .

GENE THERAPY, 2000, 7 (14) :1183-1192