Analysis of Acanthopanax Senticosus Harms for Different Parts Using Fourier Transform Infrared Spectroscopy

In the present paper, raw radix, stem and leaf and the total glycoside extract of them of Acanthopanax senticosus Harms were studied via the multi-steps IR macro-fingerprint method. The spectra of raw medicinal materials show that the spectra of radix and stem are similar and mainly show characteristic peaks of calcium oxalate and starch, whereas the characteristic peaks of calcium oxalate of leaf almost disappear and the shape of characteristic peak of starch also gets unconspicuous. The FTIR spectra of total glycoside extract of radix, stem and leaf present characteristic peaks at 1 602, 1514, 1452 cm(-1) (vibration of phenyl framework) and 1 271 cm(-1) (=C-O), respectively, therefore, the authors speculated that their mutual component is the compound of phenolic glycoside. Through observing the second derivative IR spectra of the total glycoside extract of different parts of Acanthopanax senticosus Harms, the authors found that the characteristic peak of leaf is stronger than that of radix and stem at 1 656 cm(-1)(flavone C=O), this proves that the quantity of component of flavone in the leaf is higher than that in the radix and stem. In the two-dimensional correlation spectra, the radix and stem both have five automatic peaks (vibration of phenyl framework) in 1 350-1 700 cm(-1), whereas the leaf still shows another automatic peak at 1 656 cm(-1) (flavone C=O), and this further proves that the quantity of component of flavone in the leaf is higher than that in the radix and stem.