Electrochemical Detection of Chromium(VI): Induced DNA Damage

被引：4

作者：

Jarczewska, Marta ^{[1
]}

Ziolkowski, Robert ^{[1
]}

Gorski, Lukasz ^{[1
]}

Malinowska, Elzbieta ^{[1
]}

机构：

[1] Warsaw Univ Technol, Fac Chem, Dept Microbioanalyt, Inst Biotechnol, PL-00664 Warsaw, Poland

来源：

JOURNAL OF THE ELECTROCHEMICAL SOCIETY | 2015年 / 162卷 / 12期

关键词：

IN-VITRO; ANTIOXIDATIVE PROPERTIES; BIOSENSORS; ADDUCTS; CLEAVAGE; MUTAGENICITY; MECHANISMS; FLAVONOIDS; ELECTRODE; EXTRACTS;

D O I：

10.1149/2.0401512jes

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

The DNA damage induced by chromium(VI) was examined with the use of gold disk electrodes modified with double-stranded DNA. DNA immobilization method and the pH conditions were optimized. The developed assay relies on the change of the reduction current of an external redox indicator - methylene blue (MB) before and after incubation in potassium chromate solution. The degrading effect of Cr(VI) on dsDNA layer was also observed with the use of quartz crystal microbalance (QCM) measurements. It was shown that the presence of ascorbic acid limits the degrading properties of chromium(VI), and further addition of H2O2 screens the antioxidant activity of ascorbic acid. In the proposed system, the MB current difference rises proportionally within the range of 1 to 100 mu mol L-1 of K2CrO4, while for higher concentrations the DNA layer saturation with Cr(VI) is observed. (C) 2015 The Electrochemical Society. All rights reserved.

引用

页码：B326 / B331

页数：6

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