Urinary Cell-Free DNA Quantification as Non-Invasive Biomarker in Patients with Bladder Cancer

被引：45

作者：

Brisuda, Antonin ^{[1
,2
]}

Pazourkova, Eva ^{[3
]}

Soukup, Viktor ^{[4
]}

Horinek, Ales ^{[5
,6
]}

Hrbacek, Jan ^{[1
,2
]}

Capoun, Otakar ^{[4
]}

Svobodova, Iveta ^{[5
]}

Pospisilova, Sarka ^{[5
]}

Korabecna, Marie ^{[5
]}

Mares, Jaroslav ^{[7
]}

Hanus, Tomas ^{[4
]}

Babjuk, Marek ^{[1
,2
]}

机构：

[1] Charles Univ Prague, Fac Med 2, Dept Urol, V Uvalu 84, CZ-15006 Prague, Czech Republic

[2] Univ Hosp Motol, V Uvalu 84, CZ-15006 Prague, Czech Republic

[3] Charles Univ Prague, Fac Med 1, Dept Nephrol, CZ-15006 Prague, Czech Republic

[4] Charles Univ Prague, Fac Med 1, Dept Urol, CZ-15006 Prague, Czech Republic

[5] Charles Univ Prague, Fac Med 1, Inst Biol & Med Genet, CZ-15006 Prague, Czech Republic

[6] Charles Univ Prague, Fac Med 1, Dept Med 3, CZ-15006 Prague, Czech Republic

[7] Charles Univ Prague, Fac Med 2, Dept Biol & Med Genet, CZ-15006 Prague, Czech Republic

来源：

UROLOGIA INTERNATIONALIS | 2016年 / 96卷 / 01期

关键词：

Cell-free DNA; Urinary marker; Bladder cancer; Real-time PCR; FREE SERUM DNA; MARKER; HYPERMETHYLATION; INTEGRITY;

D O I：

10.1159/000438828

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Introduction: Concentration of urinary cell-free DNA (ucfDNA) belongs to potential bladder cancer markers, but the reported results are inconsistent due to the use of various non-standardised methodologies. The aim of the study was to standardise the methodology for ucfDNA quantification as a potential non-invasive tumour biomarker. Material and Methods: In total, 66 patients and 34 controls were enrolled into the study. Volumes of each urine portion (V) were recorded and ucfDNA concentrations (c) were measured using real-time PCR. Total amounts (TA) of ucfDNA were calculated and compared between patients and controls. Diagnostic accuracy of the TA of ucfDNA was determined. Results: The calculation of TA of ucfDNA in the second urine portion was the most appropriate approach to ucfDNA quantification, as there was logarithmic dependence between the volume and the concentration of a urine portion (p = 0.0001). Using this methodology, we were able to discriminate between bladder cancer patients and subjects without bladder tumours (p = 0.0002) with area under the ROC curve of 0.725. Positive and negative predictive value of the test was 90 and 45%, respectively. Conclusion: Quantification of ucf DNA according to our modified method could provide a potential non-invasive biomarker for diagnosis of patients with bladder cancer. (C) 2015 S. Karger AG, Basel

引用

页码：25 / 31

页数：7

共 19 条

[1] Cell-free DNA levels as a prognostic marker in acute myocardial infarction [J].