Single-Cell Genomics

被引：52

作者：

Paolillo, Carmela ^{[1
]}

Londin, Eric ^{[2
]}

Fortina, Paolo ^{[3
,4
]}

机构：

[1] Univ Penn, Perelman Sch Med, Dept Clin Pathol & Lab Med Idne, Div Precis & Computat Diagnost, Philadelphia, PA 19104 USA

[2] Thomas Jefferson Univ, Computat Med Ctr, Sidney Kimmel Med Coll, Philadelphia, PA 19107 USA

[3] Thomas Jefferson Univ, Dept Canc Biol, Sidney Kimmel Med Coll, Philadelphia, PA 19107 USA

[4] Sapienza Univ, Dept Mol Med, Rome, Italy

来源：

CLINICAL CHEMISTRY | 2019年 / 65卷 / 08期

关键词：

CIRCULATING TUMOR-CELLS; MESSENGER-RNA-SEQ; CLONAL EVOLUTION; STEM-CELLS; ANTIGEN; REVEALS; HETEROGENEITY; EXPRESSION; POPULATIONS; CIRCUITS;

D O I：

10.1373/clinchem.2017.283895

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

BACKGROUND: Single-cell genomics is an approach to investigate cell heterogeneity and to identify new molecular features correlated with clinical outcomes. This approach allows identification of the complexity of cell diversity in a sample without the loss of information that occurs when multicellular or bulk tissue samples are analyzed. CONTENT: The first single-cell RNA-sequencing study was published in 2009, and since then many more studies and single-cell sequencing methods have been published. These studies have had a major impact on several fields, including microbiology, neurobiology, cancer, and developmental biology. Recently, improvements in reliability and the development of commercial single-cell isolation platforms are opening the potential of this technology to the clinical laboratory. SUMMARY: In this review we provide an overview of the current state of single-cell genomics. We describe opportunities in clinical research and medical applications. (C) 2019 American Association for Clinical Chemistry

引用

页码：972 / 985

页数：14

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