Environmental impact on age-related dynamics of karyotypical instability in plants

被引：3

作者：

Bezrukov, VF ^{[1
]}

Lazarenko, LM ^{[1
]}

机构：

[1] Taras Shevchenko Natl Univ Kiev, Dept Gen & Mol Genet, UA-01033 Kiev, Ukraine

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2002年 / 520卷 / 1-2期

关键词：

environmental impact; karyotypical instability; chromosome aberrations; transgenerational impact; aging; seeds; Allium fistulosum L;

D O I：

10.1016/S1383-5718(02)00204-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The dynamics of karyotypical instability of Allium fistulosum L. (Welsh onion) during aging of genetically homogenous seeds from plants grown in three different areas was studied. We analyzed the frequency of anaphase cells with chromosomal aberrations "damage", as a number of chromosomal aberrations per cell with aberrations, and germinating capacity, as an indicator of the 'toxic' influence of age. The seeds' aging was accompanied by an increase in karyotypical instability (increasing frequency of anaphases with aberrations) and with certain changes in the spectrum of chromosome aberrations. The clearest distinctions between old and young seeds were found for the frequency of anaphase cells with chromosome aberrations. The general level of karyotypical instability positively correlates with the age of the seeds. The regression coefficient (b) corresponds to the general tendency of karyotypical instability during seeds' senescence under storage. For 'good' (A), 'normal' (B) and 'bad' (C) conditions, the coefficients (b's) are b(A) = 0.22, b(B) = 0.46 and b(C) = 0.84 (p < 0.05 for C, and p < 0.001 for A and B). It was found that different ecological conditions of plant vegetation strongly influence age-related dynamics of chromosomal instability in the seeds obtained from these plants. Possible mechanisms of the transgenerational impact of this effect are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：113 / 118

页数：6

共 9 条

[1] Genome stability - Transgenerational mutation by radiation [J].

Dubrova, YE ;

Plumb, M ;

Gutierrez, B ;

Boulton, E ;

Jeffreys, AJ .

NATURE, 2000, 405 (6782) :37-37

[2]

Grant VJ, 1996, HEALTH CARE ANAL, V4, P76

[3] Higher plant assays for the detection of chromosomal aberrations and gene mutations - a brief historical background on their use for screening and monitoring environmental chemicals [J].

Grant, WF .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1999, 426 (02) :107-112

[4] Some aspects on radiation induced transmissible genomic instability [J].

Harms-Ringdahl, M .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1998, 404 (1-2) :27-33

[5] Transgenic plants are sensitive bioindicators of nuclear pollution caused by the Chernobyl accident [J].

Kovalchuk, I ;

Kovalchuk, O ;

Arkhipov, A ;

Hohn, B .

NATURE BIOTECHNOLOGY, 1998, 16 (11) :1054-1059

[6]

LAZARENKO LM, 1996, CYTOL GENET, V29, P72

[7] Chromosomal instability in the descendants of unirradiated surviving cells after α-particle irradiation [J].

Lorimore, SA ;

Kadhim, MA ;

Pocock, DA ;

Papworth, D ;

Stevens, DL ;

Goodhead, DT ;

Wright, EG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (10) :5730-5733

[8]

*WHO, 1985, 51 WHO

[9] Targeted cytoplasmic irradiation with alpha particles induces mutations in mammalian cells [J].

Wu, LJ ;

Randers-Pehrson, G ;

Xu, A ;

Waldren, CA ;

Geard, CR ;

Yu, ZL ;

Hei, TK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (09) :4959-4964

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