How good can cryo-EM become?

被引：122

作者：

Glaeser, Robert M. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA

来源：

NATURE METHODS | 2016年 / 13卷 / 01期

基金：

美国国家卫生研究院;

关键词：

PARTICLE ELECTRON CRYOMICROSCOPY; CRYOELECTRON MICROSCOPY; HIGH-RESOLUTION; EWALD SPHERE; PHASE-CONTRAST; LIMITATIONS; RECONSTRUCTIONS; FUTURE; NOISE;

D O I：

10.1038/nmeth.3695

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The suddenness with which single-particle cryo-electron microscopy (cryo-EM) has emerged as a method for determining high-resolution structures of biological macromolecules invites the questions, how much better can this technology get, and how fast is that likely to happen? Though we can rightly celebrate the maturation of cryo-EM as a high-resolution structure-determination tool, I believe there still are many developments to look forward to.

引用

页码：28 / 32

页数：5

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