Plasma Circulating Cell-free DNA Integrity as a Promising Biomarker for Diagnosis and Surveillance in Patients with Hepatocellular Carcinoma

被引：74
作者：
Huang, Ao [1 ,2 ]
Zhang, Xin [1 ,2 ]
Zhou, Shao-Lai [1 ,2 ]
Cao, Ya [3 ,4 ]
Huang, Xiao-Wu [1 ,2 ]
Fan, Jia [1 ,2 ,5 ]
Yang, Xin-Rong [1 ,2 ]
Zhou, Jian [1 ,2 ,6 ]
机构：
[1] Fudan Univ, Zhongshan Hosp, Liver Canc Inst, Liver Surg Dept, Shanghai 200032, Peoples R China
[2] Fudan Univ, Minist Educ, Key Lab Carcinogenesis & Canc Invas, Shanghai 200032, Peoples R China
[3] Cent South Univ, Canc Res Inst, Changsha 410078, Hunan, Peoples R China
[4] Minist Educ, Key Lab Carcinogenesis & Canc Invas, Changsha 410078, Hunan, Peoples R China
[5] Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China
[6] Fudan Univ, State Key Lab Genet Engn, Shanghai 200433, Peoples R China
来源：
JOURNAL OF CANCER | 2016年 / 7卷 / 13期
基金：
中国国家自然科学基金; 美国国家科学基金会;
关键词：
circulating cell-free DNA; cfDNA; integrity; hepatocellular carcinoma; NUCLEIC-ACIDS; CANCER; PREDICTION; BLOOD; SERUM;
D O I：
10.7150/jca.15618
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The clinical significance of circulating cell-free DNA (cfDNA) integrity as diagnostic and surveillance biomarker in hepatocellular carcinoma (HCC) was investigated and compared to that of alpha fetoprotein (AFP). Liver cancer patients had lower cfDNA integrity than those with benign diseases (P = 0.0167) and healthy individuals (P = 0.0025). Patients with HCC and non-HCC liver cancers (P = 0.7356), and patients with benign diseases and healthy individuals (P = 0.9138) had comparable cfDNA integrity respectively. cfDNA integrity increased after hepatectomy in cancer patients (P = 0.0003). The AUCs for detecting HCC by cfDNA integrity and AFP were 0.705 (P = 0.005) and 0.605 (P = 0.156), respectively. We found cfDNA integrity decreased in HCC patients and has the potential as promising biomarker for HCC diagnosis and treatment surveillance.
引用
收藏
页码：1798 / 1803
页数：6
相关论文
共 35 条
[1]   Measurement of Circulating Cell-Free DNA Levels by a Simple Fluorescent Test in Patients With Breast Cancer [J].
Agassi, Ravit ;
Czeiger, David ;
Shaked, Gad ;
Avriel, Avital ;
Sheynin, Jony ;
Lavrenkov, Konstantin ;
Ariad, Samuel ;
Douvdevani, Amos .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2015, 143 (01) :18-24
[2]   Improved sensitivity of circulating tumor DNA measurement using short PCR amplicons [J].
Andersen, Rikke Fredslund ;
Spindler, Karen-Lise Garm ;
Brandslund, Ivan ;
Jakobsen, Anders ;
Pallisgaard, Niels .
CLINICA CHIMICA ACTA, 2015, 439 :97-101
[3]  
[Anonymous], J UROL
[4]  
[Anonymous], CLIN BIOCH
[5]  
[Anonymous], 2015, P NATL ACAD SCI US
[6]   Hepatocellular carcinoma epidemiology [J].
Bosetti, Cristina ;
Turati, Federica ;
La Vecchia, Carlo .
BEST PRACTICE & RESEARCH CLINICAL GASTROENTEROLOGY, 2014, 28 (05) :753-770
[7]   Total serum DNA and DNA integrity: diagnostic value in patients with hepatitis B virus-related hepatocellular carcinoma [J].
Chen, Hui ;
Sun, Ling-Yu ;
Zheng, Hong-Qun ;
Zhang, Qi-Fan ;
Jin, Xiao-Ming .
PATHOLOGY, 2012, 44 (04) :318-324
[8]   Value of circulating cell-free DNA in diagnosis of hepatocelluar carcinoma [J].
Chen, Ken ;
Zhang, Hong ;
Zhang, Li-Na ;
Ju, Shao-Qing ;
Qi, Jing ;
Huang, Dong-Feng ;
Li, Feng ;
Wei, Qun ;
Zhang, Jing .
WORLD JOURNAL OF GASTROENTEROLOGY, 2013, 19 (20) :3143-3149
[9]   Circulating cell-free DNA in serum as a biomarker of colorectal cancer [J].
da Silva Filho, Benisio Ferreira ;
Almeida Diniz Gurgel, Ana Pavla ;
de Freitas Lins Neto, Manoel Alvaro ;
de Azevedo, Dalmo Almeida ;
de Freitas, Antonio Carlos ;
Silva Neto, Jacinto da Costa ;
Ferreira Silva, Luiz Antonio .
JOURNAL OF CLINICAL PATHOLOGY, 2013, 66 (09) :775-778
[10]   Liquid Biopsies: Genotyping Circulating Tumor DNA [J].
Diaz, Luis A. ;
Bardelli, Alberto .
JOURNAL OF CLINICAL ONCOLOGY, 2014, 32 (06) :579-+
1234