Analysis of Natural Nucleosides and Their Derivatives by Thin-Layer Chromatography

被引：3

作者：

Antonova, S. V. ^{[1
]}

Demina, N. G. ^{[1
]}

Lobanov, K. V. ^{[1
]}

Kuvaev, T. A. ^{[1
]}

Mironov, A. S. ^{[1
]}

Pushkov, A. A. ^{[1
]}

Rumiantseva, N. F. ^{[1
]}

Tyaglov, B. V. ^{[1
]}

Shakulov, R. S. ^{[1
]}

Malakhova, I. I. ^{[2
]}

Krasikov, V. D. ^{[2
]}

机构：

[1] Res Inst Genet & Select Ind Microorganisms GosNII, Moscow 117545, Russia

[2] Sci & Prod Ctr Lenchrom, St Petersburg 199004, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2016年 / 52卷 / 07期

关键词：

densitometry; HPLC; nucleosides; thin-layer chromatography; OLIGODEOXYRIBONUCLEOTIDES; SEPARATION;

D O I：

10.1134/S0003683816070024

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recently published data on the separation and quantification of natural nucleosides and some of their derivatives by thin-layer chromatography on silica gel have been summarized. The use of more than 20 mobile systems for the separation of more than 52 nucleosides and derivatives was discussed; in a few cases, the conditions for their densitometry quantitative analysis after TLC separation were considered. The works performed at GosNIIgenetika on the determination of inosine, thymidine, and acadesine with domestic Sorbfil plates were reviewed in detail.

引用

页码：714 / 721

页数：8

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