Quantification of co-trimoxazole in serum and plasma using MS/MS

被引：4

作者：

Dijkstra, Jacob A. ^{[1
]}

Alsaad, Noor S. I. ^{[1
]}

van Hateren, Kai ^{[1
]}

Ben Greijdanus ^{[1
]}

Touw, Daan J. ^{[1
,2
]}

Alffenaar, Jan-Willem C. ^{[1
]}

机构：

[1] Univ Groningen, Univ Med Ctr Groningen, Dept Clin Pharm & Pharmacol, Groningen, Netherlands

[2] Univ Groningen, Dept Pharm, Div Pharmacokinet Toxicol & Targeting, Groningen, Netherlands

来源：

BIOANALYSIS | 2015年 / 7卷 / 21期

关键词：

HIV-INFECTED PATIENTS; TRIMETHOPRIM; SULFAMETHOXAZOLE; PROPHYLAXIS; PNEUMONIA; THERAPY; HPLC;

D O I：

10.4155/bio.15.188

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Co-trimoxazole is frequently used in the prophylaxis and treatment of Pneumocystis carinii pneumonia. High plasma concentrations of sulfamethoxazole or trimethoprim are correlated with toxicity. There is, however, a large variation in PK observed which can lead to underexposure or toxicity. Results: We developed a novel LC-MS/MS method to analyze the components of co-trimoxazole, trimethoprim and sulfamethoxazole and its metabolite sulfamethoxazole-N-acetyl. This new method is expeditious due to its limited sample preprocessing and a relatively short run-time of only 3 min. Conclusion: This new method met the US FDA requirements on linearity, selectivity, precision, accuracy, matrix effects, recovery and stability and is suitable for routine analysis and future prospective studies.

引用

页码：2741 / 2749

页数：9

共 22 条

[21] SIMULTANEOUS DETERMINATION OF SULFAMETHOXAZOLE AND TRIMETHOPRIM IN RAT PLASMA BY LC-ESI-MS AND ITS APPLICATION TO A PHARMACOKINETIC STUDY [J].

Wang, Xianchuan ;

Zhang, Yuqing ;

Qin, Kuiyou ;

Nian, Siyun ;

Xiao, Tong ;

Zhang, Qingwei ;

Wang, Xianqin ;

Ma, Jianshe .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2012, 35 (07) :951-962

[22]

World Health Organization, 2006, GUID COTR PROPH HIV

← 1 2 3 →