Systems for the detection and analysis of protein-protein interactions

被引：24

作者：

Kuroda, Kouichi ^{[1
]}

Kato, Michiko ^{[1
]}

Mima, Joji ^{[1
]}

Ueda, Mitsuyoshi ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2006年 / 71卷 / 02期

关键词：

D O I：

10.1007/s00253-006-0395-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The analysis of protein-protein interactions is important for developing a better understanding of the functional annotations of proteins that are involved in various biochemical reactions in vivo. The discovery that a protein with an unknown function binds to a protein with a known function could provide a significant clue to the cellular pathway concerning the unknown protein. Therefore, information on protein-protein interactions obtained by the comprehensive analysis of all gene products is available for the construction of interactive networks consisting of individual protein-protein interactions, which, in turn, permit elaborate biological phenomena to be understood. Systems for detecting protein-protein interactions in vitro and in vivo have been developed, and have been modified to compensate for limitations. Using these novel approaches, comprehensive and reliable information on protein-protein interactions can be determined. Systems that permit this to be achieved are described in this review.

引用

页码：127 / 136

页数：10

共 83 条

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