Amperometric S-nitrosothiol sensor with enhanced sensitivity based on organoselenium catalysts

被引：28
作者：
Cha, Wansik [1 ]
Anderson, Meredith R. [1 ]
Zhang, Fenghua [1 ]
Meyerhoff, Mark E. [1 ]
机构：
[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA
来源：
BIOSENSORS & BIOELECTRONICS | 2009年 / 24卷 / 08期
关键词：
S-Nitrosothiols; Transnitrosation; Organoselenium catalyst; Amperometric nitric oxide sensor; Cellulose dialysis membrane; NITRIC-OXIDE SENSOR; ENDOTHELIAL DYSFUNCTION; SERUM-ALBUMIN; POLYMERIC FILMS; TRANSNITROSATION; BLOOD; KINETICS; PLASMA; THIOLS; LAYER;
D O I：
10.1016/j.bios.2008.12.022
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A new S-nitrosothiol (RSNO) detection strategy based on an electrochemical sensor is described for rapidly estimating levels of total RSNOs in blood and other biological samples. The sensor employs a cellulose dialysis membrane covalently modified with an organoselenium catalyst that converts RSNOs to NO at the distal tip of an amperometric NO sensor. The sensor is characterized by very low detection limits (<20 nM), good long-term stability, and can be employed for the rapid detection of total low-molecular-weight (LMW) RSNO levels in whole blood samples using a simple standard addition method. A strategy for detecting macromolecular RSNOs is also demonstrated via use of a transnitrosation reaction with added LMW thiols allowing the estimation of total RSNO levels in blood. The sensor is shown to exhibit high selectivity over nitrosamines and nitrite. Such RSNO detection is potentially useful to reveal correlation between blood RSNO levels and endothelial cell dysfunction, which often is associated with cardiovascular diseases. (C) 2008 Elsevier B.V. All rights reserved
引用
收藏
页码：2441 / 2446
页数：6
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