Enantiomeric separation of fluoxetine derivatives on polysaccharide-based chiral columns

被引：14
作者：
Li Yu [1 ]
Fa Mei-Li [1 ]
Xing Jie-Guo [1 ]
机构：
[1] Shenyang Pharmaceut Univ, Dept Analyt Chem, Sch Pharm, Shenyang 110016, Peoples R China
来源：
ARCHIV DER PHARMAZIE | 2006年 / 339卷 / 08期
关键词：
enantiomeric separation; derivatized fluoxetine; mobile phase composition; column temperature; chiral recognition mechanism;
D O I：
10.1002/ardp.200500261
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
A high performance liquid chromatography (HPLC) method employing amylose-based chiral columns (Chiralpak AD-RH and Chiralpak AD) and cellulose-based chiral columns (Chiralcel OD) as chiral stationary phases have been developed for the enantiomeric separation of fluoxetine (FLX) derivatives. The FLX was derivatized with 4-(N-chloroformylmethyl-N-methyl)amino-7-nitro-2,1,3-benzoxadiazole (NBD-COCl) and 4-(N-chloroformylmethyl-N-methyl)amino-7-N,N-dimethylaminosulfonyl-2,1,3-benzoxadiazole (DBD-COCl), respectively. Influence of the mobile phase composition and column temperature on the enantioseparation was discussed during the separation. On the basis of separation of derivatized FLX enantiomers, the paper also discussed the separation mechanism on the chiral stationary phases used.
引用
收藏
页码：461 / 465
页数：5
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