Position-dependent correlation between TBX22 exon 5 methylation and palatal shelf fusion in the development of cleft palate

被引：2

作者：

Li, Ke ^{[1
]}

Shu, Xuan ^{[1
]}

Gong, Hui ^{[2
]}

Cheng, Liuhanghang ^{[1
]}

Dong, Zejun ^{[1
]}

Shu, Shenyou ^{[1
]}

机构：

[1] Shantou Univ, Med Coll, Affiliated Hosp 2, Cleft Lip & Palate Treatment Ctr, 69 Dongxia North Rd, Jinping Dist 515041, Shantou, Peoples R China

[2] Shantou Univ, Med Coll, Affiliated Hosp 2, Dept Gynaecol, 69 Dongxia North Rd, Jinping Dist 515041, Shantou, Peoples R China

来源：

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS | 2019年 / 91卷 / 02期

基金：

美国国家科学基金会;

关键词：

DNA methylation; cleft palate; TBX22; CpG site; DNA-METHYLATION; MOLECULAR-MECHANISMS; EXPRESSION ANALYSIS; FREQUENT CAUSE; MUTATIONS; PATTERNS; GENETICS; PROMOTER; REVEALS; GENES;

D O I：

10.1590/0001-3765201920180945

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA methylation is essential for spatiotemporally-regulated gene expression in embryonic development. TBX22 (Chr X: 107667964-107688978) functioning as a transcriptional repressor affects DNA binding, sumoylation, and transcriptional repression associated with X-linked cleft palate. This study aimed to explore the relationship and potential mechanism between TBX22 exon 5 methylation and palatal shelf fusion induced by all-trans retinoic acid (ATRA). We performed DNA methylation profiling, using MethylRAD-seq, after high throughput sequencing of mouse embryos from control (n=9) and ATRA-treated (to induce cleft palate, n=9) C57BL/6J mice at embryonic gestation days(E) 13.5, 14.5 and 16.5. TBX22 exon 5 was hyper-methylated at the CpG site at E13.5 (P=0.025, log2FC=1.5) and E14.5 (P=0.011, log2FC: 1.5) in ATRA-treated, whereas methylation TBX22 exon 5 at the CpG site was not significantly different at E16.5 (P=0.808, log2FC=-0.2) between control and ATRA-treated. MSP results showed a similar trend consistent with the MethylRAD-seq results. qPCR showed the change in TBX22 exon 5 expression level negatively correlated with its TBX22 exon 5 methylation level. These results indicate that changes in TBX22 exon 5 methylation might play an important regulatory role during palatal shelf fusion, and may enlighten the development of novel epigenetic biomarkers in the treatment of CP in the future.

引用

页数：9

共 42 条

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