Long non-protein coding RNA DANCR functions as a competing endogenous RNA to regulate osteoarthritis progression via miR-577/SphK2 axis

被引:68
作者
Fan, Xiaochen [1 ]
Yuan, Jishan [1 ]
Xie, Jun [1 ]
Pan, Zhanpeng [1 ]
Yao, Xiang [1 ]
Sun, Xiangyi [2 ]
Zhang, Pin [3 ]
Zhang, Lei [2 ]
机构
[1] Jiangsu Univ, Affiliated Zhenjiang Hosp 1, Dept Orthopaed, Zhenjiang 212002, Peoples R China
[2] Nanjing Univ, Sch Med, Jinling Hosp, Dept Orthoped, Nanjing 210002, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Dept Orthoped, Jinling Clin Med Coll, Nanjing 210002, Jiangsu, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
Osteoarthritis; lncRNA DANCR; miR-577; SphK2; ceRNA; DIFFERENTIATION; PROLIFERATION; CHONDROCYTE; BIOGENESIS; PATHWAYS; DISEASE;
D O I
10.1016/j.bbrc.2018.04.130
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Long noncoding RNAs (IncRNAs) have been known to be involved in multiple diverse diseases, including osteoarthritis (OA). This study aimed to explore the role of differentiation antagonizing non-protein coding RNA (DANCR) in OA and identify the potential molecular mechanisms. The expression of DANCR in cartilage samples from patients with OA was detected using quantitative reverse transcription-polymerase chain reaction. The effects of DANCR on the viability of OA chondrocytes and apoptosis were explored using cell counting kit 8 assay and flow cytometry assay, respectively. Additionally, the interaction among DANCR, miR-577, and SphK2 was explored using dual-luciferase reporter and RIP assays. The present study found that DANCR was significantly upregulated in patients with OA. Functional assays demonstrated that DANCR inhibition suppressed the proliferation of OA chondrocytes and induced cell apoptosis. The study also showed that DANCR acted as a competitive endogenous RNA to sponge miR-577, which targeted the mRNA of SphK2 to regulate the survival of OA chondrocytes. In conclusion, the study revealed that IncRNA DANCR might promote the proliferation of OA chondrocytes and reduce apoptosis through the miR-577/SphK2 axis. Thus, IncRNA DANCR might be considered as a potential therapeutic target for OA treatment. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码:658 / 664
页数:7
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