Dynamic Metabolome Analysis Reveals the Metabolic Fate of Medium-Chain Fatty Acids in AML12 Cells

被引:26
作者
Fushimi, Tatsuya [1 ,2 ]
Izumi, Yoshihiro [2 ,3 ]
Takahashi, Masatomo [3 ]
Hata, Kosuke [3 ]
Murano, Yoshihiro [1 ]
Bamba, Takeshi [2 ,3 ]
机构
[1] Nisshin OilliO Grp Ltd, Cent Res Lab, Yokohama, Kanagawa 2358558, Japan
[2] Kyushu Univ, Grad Sch Syst Life Sci, Dept Syst Life Sci, Fukuoka 8128582, Japan
[3] Kyushu Univ, Med Inst Bioregulat, Div Metabol, Fukuoka 8128582, Japan
基金
日本科学技术振兴机构; 日本学术振兴会;
关键词
medium-chain fatty acids; ketone bodies; hepatocytes; metabolome analysis; metabolic turnover analysis; RAT-LIVER MITOCHONDRIA; LIPID-ACCUMULATION; KETONE-BODIES; CHROMATOGRAPHY; KETOGENESIS; OXIDATION; APOPTOSIS; OCTANOATE; TRIACYLGLYCEROLS; GLUCONEOGENESIS;
D O I
10.1021/acs.jafc.0c04723
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Several studies in hepatocyte cell lines reported that medium-chain fatty acids (MCFAs) with 6-12 carbons showed different metabolic properties from long-chain fatty acids (LCFAs). However, these studies reported unclear effects of different fatty acid molecules on hepatocyte metabolism. This study is aimed to capture the metabolic kinetics of MCFA assimilation in AML12 cells treated with octanoic acid (FA 8:0), decanoic acid (FA 10:0), or lauric acid (FA12:0) [LCFA; oleic acid (FA 18:1)] via metabolic profiling and dynamic metabolome analysis with C-13-labeling. The concentrations of total ketone bodies in the media of cells treated with FA 8:0 or FA 10:0 were 3.22- or 3.69-fold higher than those obtained with FA 18:1 treatment, respectively. FA 12:0 treatment did not significantly increase ketone body levels compared to DMSO treatment (control), whereas FA 12:0 treatment increased intracellular triacylglycerol (TG) levels 15.4 times compared to the control. Metabolic profiles of FA 12:0treated samples differed from those of the FA 8:0-treated and FA 10:0-treated samples, suggesting that metabolic assimilation of MCFAs differed significantly depending on the MCFA type. Furthermore, the dynamic metabolome analysis clearly revealed that FA 8:0 was rapidly and quantitatively oxidized to acetyl-CoA and assimilated into ketone bodies, citrate cycle intermediates, and glucogenic amino acids but not readily into TGs.
引用
收藏
页码:11997 / 12010
页数:14
相关论文
共 69 条
[11]  
Dayrit F., 2014, PHILIPP J SCI, V143, P157
[12]   The Properties of Lauric Acid and Their Significance in Coconut Oil [J].
Dayrit, Fabian M. .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 2015, 92 (01) :1-15
[13]   Palmitic acid induces endoplasmic reticulum stress in AML12 liver cells [J].
Demirel, Tugce ;
Batirel, Saime .
MARMARA MEDICAL JOURNAL, 2018, 31 (01) :40-46
[14]   Lipid signaling and lipotoxicity in metaflammation: indications for metabolic disease pathogenesis and treatment [J].
Ertunc, Meric Erikci ;
Hotamisligil, Gokhan S. .
JOURNAL OF LIPID RESEARCH, 2016, 57 (12) :2099-2114
[15]   Role of fat-derived substrates in the regulation of gluconeogenesis during fasting [J].
Fery, F ;
Plat, L ;
Melot, C ;
Balasse, EO .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1996, 270 (05) :E822-E830
[16]   Long-chain fatty acids induce lipid droplet formation in a cultured human hepatocyte in a manner dependent of acyl-CoA synthetase [J].
Fujimoto, Yasuyuki ;
Onoduka, Jun ;
Homma, Koichi J. ;
Yamaguchi, Shinji ;
Mori, Masahiro ;
Higashi, Yusuke ;
Makita, Minoru ;
Kinoshita, Tetsuaki ;
Noda, Jun-ichi ;
Itabe, Hiroyuki ;
Takano, Tatsuya .
BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2006, 29 (11) :2174-2180
[17]   Esterification of free fatty acids in adipocytes: a comparison between octanoate and oleate [J].
Guo, W ;
Choi, JK ;
Kirkland, JL ;
Corkey, BE ;
Hamilton, JA .
BIOCHEMICAL JOURNAL, 2000, 349 :463-471
[18]   Octanoate inhibits triglyceride synthesis in 3T3-L1 and human adipocytes [J].
Guo, W ;
Lei, TG ;
Wang, T ;
Corkey, BE ;
Han, JR .
JOURNAL OF NUTRITION, 2003, 133 (08) :2512-2518
[19]   Modulation of adipocyte lipogenesis by octanoate: involvement of reactive oxygen species [J].
Guo, Wen ;
Xie, Weisheng ;
Han, Jianrong .
NUTRITION & METABOLISM, 2006, 3 (1)
[20]   Promising Effect of a New Ketogenic Diet Regimen in Patients with Advanced Cancer [J].
Hagihara, Keisuke ;
Kajimoto, Katsufumi ;
Osaga, Satoshi ;
Nagai, Naoko ;
Shimosegawa, Eku ;
Nakata, Hideyuki ;
Saito, Hitomi ;
Nakano, Mai ;
Takeuchi, Mariko ;
Kanki, Hideaki ;
Kagitani-Shimono, Kuriko ;
Kijima, Takashi .
NUTRIENTS, 2020, 12 (05)