Myeloid-derived suppressor cells in human peripheral blood: Optimized quantification in healthy donors and patients with metastatic renal cell carcinoma

被引：19

作者：

Floercken, Anne ^{[1
,2
]}

Takvorian, Anna ^{[1
,2
]}

Singh, Anju ^{[1
,2
]}

Gerhardt, Anne ^{[1
,2
]}

Ostendorf, Benjamin Nils ^{[1
,2
]}

Doerken, Bernd ^{[1
,2
]}

Pezzutto, Antonio ^{[3
]}

Westermann, Joerg ^{[1
,2
]}

机构：

[1] Charitei Univ Med, Dept Hematol Oncol & Tumor Immunol, Campus Virchow Klinikum, D-13353 Berlin, Germany

[2] Labor Berlin Charite Vivantes GmbH, D-13353 Berlin, Germany

[3] Charite Univ Med Berlin, Dept Hematol Oncol & Tumor Immunol, Campus Benjamin Franklin, D-12200 Berlin, Germany

来源：

IMMUNOLOGY LETTERS | 2015年 / 168卷 / 02期

关键词：

Myeloid-derived suppressor cells; Renal cell carcinoma; Flow cytometry; CANCER-PATIENTS; T-CELLS; ACTIVATED GRANULOCYTES; INTERFERON-ALPHA; DENDRITIC CELLS; SUNITINIB; MECHANISM; EXPRESSION; THERAPIES; APOPTOSIS;

D O I：

10.1016/j.imlet.2015.10.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Induction of myeloid-derived suppressor cells is an important mechanism leading to tolerance against tumors. Phenotypic characterization of MDSC has been established and heterogeneous populations with monocytic or granulocytic features have been characterized. Increased levels of MDSC have been described in metastatic renal cell carcinoma and seem to correlate with an adverse outcome. As MDSC constitute only small populations in peripheral blood of cancer patients, it is highly important to achieve technically optimized conditions for quantification. Different cell preparation techniques - besides freezing and thawing - are potential sources of substantial variation. Our study was focused on an optimized quantification of MDSC in pB of healthy donors and patients with mRCC, in whom major technical sources of variation were analyzed. Whole blood and peripheral blood mononuclear cells were used for the flow cytometric quantification of MDSC in the pB of mRCC patients and healthy donors. We compared (1) analysis in whole blood vs. PBMC after Ficoll gradient centrifugation and (2) immediate analysis after blood drawing vs. analysis one day later. Finally, in order to evaluate our optimized technical approach, pB of 15 patients with histologically confirmed mRCC under treatment with either sunitinib or sorafenib was analyzed. No difference in the number of MDSC was observed after analysis in whole blood vs. PBMC. In contrast, the time point of analysis was a source of substantial variation (one day later vs. immediate analysis after blood drawing). In conclusion, for optimal analysis of MDSC, immediate analysis of whole blood after blood drawing rather than one day later seems to be most appropriate under the aspect of practical feasibility and reliability. Using this method, we were able to confirm both (a) increased numbers of MDSC in patients with mRCC and (b) a decrease of MDSC under sunitinib therapy. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：260 / 267

页数：8

共 37 条

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