Microbial Communities in Chesapeake & Ohio Canal National Historical Park and Their Function as Indicators of Water Quality

被引:3
作者
Yu, Hui [1 ]
Khan, Asad U. [2 ]
Subramanian, Srishty [1 ]
Vesper, Dorothy [3 ]
Van Aken, Benoit [4 ]
机构
[1] Temple Univ, Dept Civil & Environm Engn, Philadelphia, PA 19122 USA
[2] McCormick Taylor Inc, Mt Laurel, NJ USA
[3] West Virginia Univ, Dept Geol & Geog, Morgantown, WV 26506 USA
[4] George Mason Univ, Dept Chem & Biochem, 4400 Univ Dr, Fairfax, VA 22030 USA
关键词
Microbial ecology; karst water; metagenomics; microbial diversity; springs; caves; BACTERIAL COMMUNITY; RIVER; DIVERSITY; MICROORGANISMS; VARIABILITY; SEDIMENT; DYNAMICS; BIOFILM; SULFATE; IMPACT;
D O I
10.1080/01490451.2019.1612969
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The objective of this study was to establish meaningful relationships between prokaryotic community profiles and water quality parameters in different water bodies (spring, stream, cave, and mine) in the middle reach of the Chesapeake & Ohio Canal National Historic Park (C&O), Maryland. The microbial profiles in the water samples were determined using metagenomic analysis. The relationships between microbial phylogenetic profiles and water quality parameters were investigated using principal component analysis (PCA) and redundancy analysis (RDA). The most abundant phyla identified in most samples were Proteobacteria (55.4%), Bacteroidetes (12.3%), Actinobacteria (10.6%), Firmicutes (2.4%), Planktomycetes (1.8%), Verrucomicrobia (1.5%), Chloroflexi (1.5%), and Acidobacteria (1.3%), which are major bacterial and archaeal groups typically observed in natural freshwater environments. PCA showed that water chemistry was determined primarily by the geology of the site and the type of water source (i.e., spring, stream, cave, or mine). Most samples located in carbonate formations correlated with high alkalinity, inorganic carbon, and calcium, representing the typical karstic geochemistry. RDA shows that pH, electrical conductivity, temperature and nutrients including nitrate, phosphate, and sulfate, were significant determinants of the microbial ecology.
引用
收藏
页码:673 / 682
页数:10
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