Pyruvate prevents aging of mouse oocytes

被引：50
作者：
Liu, Na [1 ]
Wu, Yan-Guang [1 ]
Lan, Guo-Cheng [1 ]
Sui, Hong-Shu [1 ]
Ge, Li [1 ]
Wang, Jun-Zu [1 ]
Liu, Yong [1 ]
Qiao, Tian-Wu [1 ]
Tan, Jing-He [1 ]
机构：
[1] Shandong Agr Univ, Lab Anim Reprod & Embryol, Coll Anim Sci & Vet Med, Tai An 271018, Shandong, Peoples R China
来源：
REPRODUCTION | 2009年 / 138卷 / 02期
基金：
中国国家自然科学基金;
关键词：
IN-VITRO MATURATION; HISTONE DEACETYLASE INHIBITOR; CORTICAL GRANULE EXOCYTOSIS; CELL-CYCLE PROGRESSION; AMINO-ACID-METABOLISM; MEIOTIC MATURATION; PARTHENOGENETIC ACTIVATION; PROMOTING FACTOR; PORCINE OOCYTES; MORPHOLOGICAL-CHANGES;
D O I：
10.1530/REP-09-0122
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Inhibiting oocyte aging is important not only for healthy reproduction but also for the success of assisted reproduction techniques. Although our previous studies showed that cumulus cells accelerated aging of mouse oocytes, the underlying mechanism is unknown. The objective of this paper was to study the effects of pyruvate and cumulus cells on mouse oocyte aging. Freshly ovulated mouse cumulus-oocyte complexes (COCs) or cumulus-denuded oocytes (Dos) were cultured in Chatot-Ziomek-Bavister (CZB) medium or COC-conditioned CZB medium supplemented with different concentrations of pyruvate before being examined for aging signs and developmental potential. Pyruvate supplementation to CZB medium decreased rates of ethanol-induced activation in both COCs and Dos by maintaining their maturation-promoting factor activities, but more pyruvate was needed for COCs than for Dos. Addition of pyruvate to the COC-conditioned CZB also alleviated aging of Dos. Observations on cortical granules, level of BCL2 proteins, histone acetylation, intracellular concentration of glutathione, and embryo development all confirmed that pyruvate supplementation inhibited aging of mouse oocytes. it is concluded that the aging of mouse oocytes, facilitated by culture in COCs, can be partially prevented by the addition of pyruvate to the culture medium. Reproduction (2009) 138 223-234
引用
收藏
页码：223 / 234
页数：12
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