A quick assay for Na+-K+-ATPase specific activity

被引：0
|
作者：
Sarkar, PK [1 ]
机构：
[1] Rutgers State Univ, Dept Biol, Camden, NJ 08033 USA
来源：
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES | 2002年 / 57卷 / 5-6期
关键词：
Na+-K+-ATPase; synaptosome; protein assay;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The method describes a simultaneous determination of inorganic phosphate (Pi) and protein content from a reaction mixture used for assay of adult rat cerebrocortical synaptosomal membrane Na+-K+-ATPase specific activity. The present method is more convenient, accurate and quicker compared to the existing methods for the determination of Na+-K+-ATPase activity. It also eliminates the possible errors in protein estimation by other classical methods in brain, which have a high lipid content.
引用
收藏
页码：562 / 564
页数：3
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