Polymorphisms in FTO and TCF7L2 genes of Euro-Brazilian women with gestational diabetes

Objective: To investigate the association between fat mass and obesity-associated (FTO) gene polymorphisms rs8050136C > A and rs9939609T > A, and transcription factor 7-like 2 (TCF7L2) gene polymorphisms rs12255372G > T and rs7903146C > T, in a sample group of pregnant Euro-Brazilian women with or without gestational diabetes mellitus (GDM). Methods: Subjects were classified as either healthy pregnant control (n = 200) or GDM (n = 200) according to the 2010 criteria of the American Diabetes Association. The polymorphisms were genotyped using fluorescent probes (TaqMan (R)). Results: All groups were in the Hardy-Weinberg equilibrium. The genotype and allele frequencies of the examined polymorphisms did not exhibit significant difference (P > 0.05) between the groups. In the healthy and GDM pregnant women groups, the A-allele frequencies (95% CI) of FTO polymorphisms rs8050136 and rs9939609 were 39% (34-44%); 38% (33-43%) and 40% (35-45%); 41% (36-46%), respectively; and the T-allele frequencies of TCF7L2 polymorphisms rs12255372 and rs7903146 were 30% (26-35%), 32% (27-37%) and 29% (25-34%), 36% (31-41%), respectively. Conclusion: The examined polymorphisms were not associated with GDM in the Euro-Brazilian population studied. (C) 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.