An immunosensing device based on inhibition of mediator's faradaic process for early diagnosis of prostate cancer using bifunctional nanoplatform reinforced by carbon nanotube

被引:30
作者
Farzin, Leila [1 ]
Sadjadi, Sodeh [2 ]
Shamsipur, Mojtaba [3 ]
Sheibani, Shahab [1 ]
机构
[1] Nucl Sci & Technol Res Inst, Radiat Applicat Res Sch, Tehran, Iran
[2] Nucl Sci & Technol Res Inst, Mat & Nucl Fuel Sch, Tehran, Iran
[3] Razi Univ, Dept Chem, Kermanshah, Iran
关键词
Histidine-rGO; PSA; Saliva; Serum; Signal-off immunosensor; LIQUID-FUNCTIONALIZED GRAPHENE; ELECTROCHEMICAL DETECTION; SENSITIVE DETECTION; GOLD NANOPARTICLES; ANTIGEN; SIGNAL; NANOCOMPOSITE; PLATFORM; BIOSENSORS; PSA;
D O I
10.1016/j.jpba.2019.05.008
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this study, a simple and lable-free voltammetric immunosensor was successfully developed for the ultrasensitive detection of prostate specific antigen (PSA). To do this, multiwalled carbon nanotube (MWCNT)/L-histidine functionalized reduced graphene oxide (His-rGO) was demonstrated as a bifunctional nanoplatform for covalently attaching thionine redox indicator and anti-PSA antibody (Ab). The MWCNT enhanced electrical conductivity and facilitated the electron transfer between thionine and the glassy carbon electrode. While, the presence of anti-PSA antibody blocked the electron transfer of thionine and decreased redox signals. The principle response of proposed immunosensor was based on the selective interaction of PSA with thionine-NH2-GO-COOH-Ab. This selective interaction led to further decrease of response current of attached electrochemical probe. The liner calibration curve for tumor marker determination was 10 fg mL(-1)-20 ng mL(-1) (R-2 = 0.996). Under optimized conditions, the immunosensor was able to selectively detect PSA with a limit of detection (LOD) of 2.8 fg mL(-1) at 3 sigma. The relative standard deviations (RSDs) for single-electrode repeatability and electrode-to-electrode reproducibility were less than 2.9% and 5.7% (n = 5), respectively. Furthermore, the as-proposed immunosensor showed excellent performance in detection of PSA in the human serum and saliva samples, which implies that the current strategy has a promising feature for the clinical assessment of tumor marker status in patients with prostate cancer. Published by Elsevier B.V.
引用
收藏
页码:259 / 267
页数:9
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