Production of Brazilian human norovirus VLPs and comparison of purification methods

被引：6

作者：

da Costa Lamounier, Thais Alves ^{[1
]}

de Oliveira, Layssa Miranda ^{[2
]}

de Camargo, Brenda Rabello ^{[2
]}

Rodrigues, Kelly Barreto ^{[2
]}

Noronha, Eliane Ferreira ^{[2
]}

Ribeiro, Bergmann Morais ^{[2
]}

Nagata, Tatsuya ^{[2
]}

机构：

[1] Univ Brasilia, Dept Farmacia, BR-72220900 Brasilia, DF, Brazil

[2] Univ Brasilia, Dept Biol Celular, BR-72220900 Brasilia, DF, Brazil

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2015年 / 46卷 / 04期

关键词：

Norwalk virus; VLP; ion-exchange chromatography; VIRUS-LIKE PARTICLES; ACUTE GASTROENTERITIS; CAPSID PROTEIN; STABILITY; VP1;

D O I：

10.1590/S1517-838246420140925

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.

引用

页码：1265 / 1268

页数：4

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