A single vector cloning, mutagenesis and expression system

被引：3

作者：

Towler, EM ^{[1
]}

Stebbins, JW ^{[1
]}

Debouck, C ^{[1
]}

机构：

[1] SMITHKLINE BEECHAM PHARMACEUT,DEPT MOL GENET,KING OF PRUSSIA,PA 19406

来源：

GENE | 1996年 / 183卷 / 1-2期

关键词：

Escherichia coli; phagemid; M13;

D O I：

10.1016/S0378-1119(96)00489-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We describe a multipurpose Escherichia coli vector, pOTSflblue, that can be utilized for high efficiency subcloning, epitope-tagged protein overexpression, authentic protein overexpression and efficient mutagenesis.

引用

页码：259 / 263

页数：5

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