Engineering bone tissue using human dental pulp stem cells and an osteogenic collagen-hydroxyapatite-poly (L-lactide-co-ε-caprolactone) scaffold

被引：60
作者：
Akkouch, Adil [1 ,2 ,3 ]
Zhang, Ze [2 ,3 ]
Rouabhia, Mahmoud [1 ]
机构：
[1] Univ Laval, Fac Dent, Grp Rech Ecol Buccale, Quebec City, PQ, Canada
[2] Univ Laval, Dept Surg, Quebec City, PQ, Canada
[3] CHUQ, St Francois dAssise Hosp, Res Ctr, Quebec City, PQ, Canada
来源：
JOURNAL OF BIOMATERIALS APPLICATIONS | 2014年 / 28卷 / 06期
基金：
加拿大自然科学与工程研究理事会; 加拿大健康研究院;
关键词：
Human dental pulp stem cells; differentiation; biomaterial; poly(lactide-co--caprolactone); collagen; hydroxyapatite; bone regeneration; IN-VITRO; CALCIUM-PHOSPHATE; GENE-EXPRESSION; DIFFERENTIATION; VIVO; DEFECTS; REGENERATION; ASSOCIATION; OSTEOBLASTS; POPULATION;
D O I：
10.1177/0885328213486705
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The aim of this study was to design a new natural/synthetic bioactive bone scaffold for potential use in bone replacement applications. We developed a tri-component osteogenic composite scaffold made of collagen (Coll), hydroxyapatite (HA) and poly(l-lactide-co--caprolactone) (PLCL). This Coll/HA/PLCL composite scaffold was combined with human osteoblast-like cells obtained by differentiation of dental pulp stem cells (DPSCs) to engineer bone tissue invitro. Results show that the 3D Coll/HA/PLCL composite scaffold was highly porous, thereby enabling osteoblast-like cell adhesion and growth. Cultured in the Coll/HA/PLCL scaffold, the osteoblast-like cells expressed different osteogenic genes, produced alkaline phosphatase and formed nodules more than did PLCL alone. Micro-CT analyses revealed a significant (30%) increase of tissue mineralisation on the surface as well as inside of the Coll/HA/PLCL scaffold, thus confirming its effectiveness as a bone regeneration platform.
引用
收藏
页码：922 / 936
页数：15
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