Near-infrared, dual-ratiometric fluorescent label for measurement of pH

被引:26
|
作者
Povrozin, Yevgen A. [3 ]
Markova, Larysa I. [3 ]
Tatarets, Anatoliy L. [3 ]
Sidorov, Vadim I. [3 ]
Terpetschnig, Ewald A. [1 ,2 ]
Patsenker, Leonid D. [1 ,3 ]
机构
[1] SETA BioMed, Urbana, IL 61802 USA
[2] ISS, Champaign, IL 61822 USA
[3] Natl Acad Sci Ukraine, SSI Inst Single Crystals, UA-61001 Kharkov, Ukraine
关键词
NIR fluorescent probe; Ratiometric pH measurement; Fluorescence lifetime; BIOLOGICAL APPLICATIONS; SUCCINIMIDYL ESTERS; CYANINE DYES; LIFETIME; SPECTROSCOPY; REAGENTS; PROBES;
D O I
10.1016/j.ab.2009.03.047
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We describe the spectral properties of an amine-reactive, pH-sensitive, long-wavelength ratiometric fluorescent label having a pK(a) in the physiological pH range. The label exhibits its main absorption and emission in the near-infrared (NIR) region. On deprotonation, a blue shift of the excitation maximum is observed. Importantly, both the protonated and deprotonated forms of the label are fluorescent, with the deprotonated form having an extremely large Stokes shift of more than 100 nm. The spectral and photophysical properties of this pH label are compared with the Properties of the protein-conjugated forms. Due to the observed pK(a) shift to the acidic pH range upon conjugation to proteins, such labels are ideal for Studying phagocytic events and their regulation by drugs and/or environmental factors. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码:136 / 140
页数:5
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