A supersandwich fluorescence in situ hybridization strategy for highly sensitive and selective mRNA imaging in tumor cells

被引：22

作者：

Huang, Jin ^{[1
]}

Wang, He ^{[1
]}

Yang, Xiaohai ^{[1
]}

Yang, Yanjing ^{[1
]}

Quan, Ke ^{[1
]}

Ying, Le ^{[1
]}

Xie, Nuli ^{[1
]}

Ou, Min ^{[1
]}

Wang, Kemin ^{[1
]}

机构：

[1] Hunan Univ, Key Lab Bionanotechnol & Mol Engn Hunan Prov, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 02期

基金：

对外科技合作项目（国际科技项目）; 中国国家自然科学基金;

关键词：

ROLLING-CIRCLE AMPLIFICATION; MITOCHONDRIAL-DNA; KINASE; CANCER;

D O I：

10.1039/c5cc08503a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a supersandwich fluorescence in situ hybridization (SFISH) strategy for highly sensitive and selective in situ visualization of mRNA expression patterns at the single-cell level. This strategy uses two fluorophore-labeled signal probes to generate a supersandwich product, which in turn generates numerous signal probes located at the target mRNA position, resulting in the in situ fluorescence signal amplification.

引用

页码：370 / 373

页数：4

共 32 条

[1]

Alwine J C, 1979, Methods Enzymol, V68, P220

[2] Large heterogeneity of mitochondrial DNA transcription and initiation of replication exposed by single-cell imaging [J].

Chatre, Laurent ;

Ricchetti, Miria .

JOURNAL OF CELL SCIENCE, 2013, 126 (04) :914-926

[3] Combination of multiple mRNA markers (PTTG1, survivin, UbcH10 and TK1) in the diagnosis of Taiwanese patients with breast cancer by membrane array [J].

Chen, Chung-Chi ;

Chang, Tsai-Wang ;

Chen, Fang-Ming ;

Hou, Ming-Feng ;

Hung, Sung-Yu ;

Chong, Inn-Wen ;

Lee, Su-Chen ;

Zhou, Tian-Hong ;

Lin, Shiu-Ru .

ONCOLOGY, 2006, 70 (06) :438-446

[4] Key aspects of analyzing microarray gene-expression data [J].

Chen, James J. .

PHARMACOGENOMICS, 2007, 8 (05) :473-482

[5] Next-Generation in Situ Hybridization Chain Reaction: Higher Gain, Lower Cost, Greater Durability [J].

Choi, Harry M. T. ;

Beck, Victor A. ;

Pierce, Niles A. .

ACS NANO, 2014, 8 (05) :4284-4294

[6] Programmable in situ amplification for multiplexed imaging of mRNA expression [J].

Choi, Harry M. T. ;

Chang, Joann Y. ;

Trinh, Le A. ;

Padilla, Jennifer E. ;

Fraser, Scott E. ;

Pierce, Niles A. .

NATURE BIOTECHNOLOGY, 2010, 28 (11) :1208-U103

[7]

Couzin J, 2006, SCIENCE, V313, P1559

[8] Altered Mitochondrial Function, Mitochondrial DNA and Reduced Metabolic Flexibility in Patients With Diabetic Nephropathy [J].

Czajka, Anna ;

Ajaz, Saima ;

Gnudi, Luigi ;

Parsade, Chandani Kiran ;

Jones, Peter ;

Reid, Fiona ;

Malik, Afshan N. .

EBIOMEDICINE, 2015, 2 (06) :499-512

[9] Functional roles of superoxide and hydrogen peroxide generated by mitochondrial DNA mutation in regulating tumorigenicity of HepG2 cells [J].

Gao, Wen ;

Xu, Kehua ;

Li, Ping ;

Tang, Bo .

CELL BIOCHEMISTRY AND FUNCTION, 2011, 29 (05) :400-407

[10] A Highly Sensitive Target-Primed Rolling Circle Amplification (TPRCA) Method for Fluorescent in Situ Hybridization Detection of MicroRNA in Tumor Cells [J].

Ge, Jia ;

Zhang, Liang-Liang ;

Liu, Si-Jia ;

Yu, Ru-Qin ;

Chu, Xia .

ANALYTICAL CHEMISTRY, 2014, 86 (03) :1808-1815

← 1 2 3 4 →