Development of an enzyme-linked immunosorbent assay for octylphenol

被引：13

作者：

Li, Zhuokun ^{[1
]}

Liu, Liqiang ^{[1
]}

Song, Shanshan ^{[1
]}

Guo, Shidong ^{[1
]}

Kuang, Hua ^{[1
]}

Xu, Chuanlai ^{[1
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Sch Food Sci & Technol, Wuxi, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2014年 / 25卷 / 03期

基金：

中国国家自然科学基金;

关键词：

antibody; octylphenol; monoclonal; ELISA; PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; ANTIBODY-BASED METHODS; ALKYLPHENOL POLYETHOXYLATES; NONIONIC SURFACTANTS; SEWAGE SLUDGES; WATER SAMPLES; NONYLPHENOL; 4-NONYLPHENOL; ETHOXYLATES;

D O I：

10.1080/09540105.2013.821597

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative analysis of octylphenol. A linear carboxylated analogue of 4-nonylphenol was synthesised and characterised as hapten. The hapten was coupled to the carrier protein to produce a specific antibody. An indirect competitive ELISA method was established based on the polyclonal antibody, which exhibited an IC50 value of 51 ng/mL for octylphenol. A monoclonal antibody was produced with an IC50 value of 76 ng/mL for octylphenol. The immunoassay used with the monoclonal antibodies was applied to analyse water samples from Tai Lake in China.

引用

页码：397 / 410

页数：14

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