New Support for High-Performance Liquid Chromatography Based on Silica Coated with Alumina Particles

被引：12
作者：
Silveira, Jose Leandro R. [1 ]
Dib, Samia R. [1 ]
Faria, Anizio M. [1 ]
机构：
[1] Univ Fed Uberlandia, Quim FACIP, Grp Pesquisas Cromatog, BR-38304402 Ituiutaba, Brazil
来源：
ANALYTICAL SCIENCES | 2014年 / 30卷 / 02期
关键词：
Aluminized silica; chromatographic supports; RPLC; stability; stationary phases; STATIONARY PHASES; SOL-GEL; NANOPARTICLES; STABILITY; ZIRCONIA; SPHERES;
D O I：
10.2116/analsci.30.285
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new material based on silica coated with alumina nanoparticles was proposed for use as a chromatographic support for reversed-phase high-performance liquid chromatography. Alumina nanoparticles were synthesized by a sol-gel process in reversed micelles composed of sodium bis(2-ethylhexyl)sulfosuccinate, and the support material was formed by the self-assembly of alumina layers on silica spheres. Spectroscopic and Si-29 nuclear magnetic resonance results showed evidence of chemical bonds between the alumina nanoparticles and the silica spheres, while morphological characterizations showed that the aluminized silica maintained the morphological properties of silica desired for chromatographic purposes after alumina incorporation. Stability studies indicated that bare silica showed high dissolution (similar to 83%), while the aluminized silica remained practically unchanged (99%) after passing one liter of the alkaline mobile phase, indicating high stability under alkaline conditions. The C-18 bonded aluminized silica phase showed great potential for use in high-performance liquid chromatography to separate basic molecules in the reversed-phase mode.
引用
收藏
页码：285 / 291
页数：7
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