The TLR4 Agonist Vaccine Adjuvant, GLA-SE, Requires Canonical and Atypical Mechanisms of Action for TH1 Induction

The Toll-like receptor 4 agonist glucopyranosyl lipid adjuvant formulated in a stable emulsion (GLA-SE) promotes strong T(H)1 and balanced IgG1/IgG2 responses to protein vaccine antigens. This enhanced immunity is sufficient to provide protection against many diseases including tuberculosis and leishmaniasis. To better characterize the adjuvant action it is important to understand how the different cytokines and transcription factors contribute to the initiation of immunity. In the present study using T-bet(-/-) and IL-12(-/-) mice and a blocking anti-IFN alpha R1 monoclonal antibody, we define mechanisms of adjuvant activity of GLA-SE. In accordance with previous studies of TLR4 agonist based adjuvants, we found that T(H)1 induction via GLA-SE was completely dependent upon T-bet, a key transcription factor for IFN Upsilon production and T(H)1 differentiation. Consistent with this, deficiency of IL-12, a cytokine canonical to T(H)1 induction, ablated T(H)1 induction via GLA-SE. Finally we demonstrate that the innate immune response to GLA-SE, including rapid IFN Upsilon production by memory CD8+ T cells and NK cells, was contingent on type I interferon, a cytokine group whose association with T(H)1 induction is contextual, and that they contributed to the adjuvant activity of GLA-SE.